Quote extraction and attribution is the task of automatically extracting quotes from text and attributing each quote to its correct speaker. The present state-of-the-art system uses gold standard information from previous decisions in its features, which, when removed, results in a large drop in performance. We treat the problem as a sequence labelling task, which allows us to incorporate seque...

Large water-soluble gold clusters are used for the labelling of biological material to visualize the posi­ tion of reactive sites by electron microscopy [1]. In most investigations of this type a commercial prod­ uct was used, for which little chemical information has been published (for review see [9]). Large gold clusters can be prepared using phosphines as lig­ ands [2, 3] or more easily wit...

The use of the colloidal-gold technique in electron microscopy immunocytochemistry has provided important information on the in situ localisation of intracellular antigens. We have developed a post-embedding technique for prolactin localisation on resin-embedded human pituitary tissue sections by the use of the protein-A gold conjugate. Human pituitary tissue obtained at autopsy was processed f...

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Adhalin and alpha-dystroglycan are two components of a complex of proteins that, in conjunction with dystrophin, provide a link between the subsarcolemmal cytoskeleton and the basal lamina of the extracellular matrix of skeletal muscle. In the absence of dystrophin, in Duchenne muscular dystrophy (DMD) and the mdx mouse, levels of adhalin, alpha-dystroglycan and other components of the complex,...

The distribution of DNA in Ehrlich tumour cell nucleoli was investigated by means of an immunocytochemical approach involving a monoclonal antibody directed against double- and single-stranded DNA. Immunolabelling was performed either before or after the embedding process. The postembedding labelling method allows better ultrastructural preservation than the preembedding labelling method. In pa...

In five strains of Mycoplasma gallisepticum, a protein with a molecular mass of about 40 kDa was detected by immunoblotting with anti-pig brain tubulin polyclonal and monoclonal antibodies. In eight other mycoplasma species similarly tested no reaction was observed. Thin serial sections of M. gallisepticum and Acholeplasma laidlawii cells examined by transmission electron microscopy revealed a ...