The aim of the present study was to elucidate the mode of action of goat bulbourethral lipase (BUSgp60 lipase) previously identified as responsible for the deterioration of goat sperm viability in skimmed milk-based extenders. Milk fractions were purified by micro- and ultrafiltration and characterized by SDS-PAGE, thin layer chromatography, triglyceride quantitative analysis and by their abili...

The ability to cryopreserve spermatozoa from all of the domestic species is challenging. Even though all of the cells must endure similar physical stresses associated with the cryopreservation processes, sperm from the different species are very different in size, shape and lipid composition, all of which affect cryosurvival. Thus, when a cryopreservation protocol has been optimized for sperm o...

Aims: Determine the motility of spermatozoa in fresh goat semen after being given turmeric extract.
 Study Design: Randomized Block Design.
 Place and Duration Study: Sample: Galang, Deli Serdang Regency, Indonesia, between December 2022 January 2023.
 Methodology: Giving extract to once a day. The treatment included adding 0ml, 25 ml, 50 75 100 ml extract. parameter observed was...

the aim of this study was to compare the effects of using different levels of royal jelly in semen extender on viatal characteristics of goat sperm after freezing. semen was collected from four adult bucks twice a week and samples pooled together. sperm samples (six repeats) containing royal jelly in four levels (0.5, 1, 1.5 and 2%) had been frozen. after semen samples freezing – were thawed an...

Background: This study was carried out to evaluate the protective effects of a cell permeable antioxidant, Manganese (III) meso-tetrakis (N-ethylpyridinium-2-yl) porphyrin chloride (MnTE), alone or in combination with different concentration of Catalase on motility of frozenthawed goat semen. Materials and Methods: Fresh semen ejaculates were collected with artificial vagina from 3 goats, mixed...

The aim of this study was to cryopreserve and evaluate frozen-thawed semen parameters. Electroejaculator was used to collect semen from six bucks. Semen samples were divided into two parts. In one part, seminal plasma was removed and was not in the other one. Both semen sample parts were extended with egg-yolk Tris extender. Semen was thereafter cryopreserved in straws using liquid nitrogen. Da...

South African indigenous bucks are raised under extensive conditions and are more likely to reject artificial vagina (AV) due to their limited contact with human beings, as they are less handled. The purpose of the study was to compare goat semen collection techniques used for goats based on semen traits of South African indigenous bucks during the natural breeding season. A total of eight Sout...

In this study, the production of transgenic goats using sperm to integrate exogenous DNA and artificial insemination (AI) was carried out and the technical protocols for sperm-mediated gene transfer (SMGT) in the goat were optimized. The standard sperm parameters and the ability to bind foreign genes were assessed to select suitable sperm donor bucks. A total of 134 oestrous does were divided i...

Background Sexing sperm is an important biotechnical that enables improve animal production, with achievement of maximum animal breeding. However, all sex semen methodologies can promote irreparable damages to sperm cell, which compromise their fertility [1]. Among the main causes of injures to the sperm are the reactive oxygen species, which have their production intensified during semen manip...