introduction: the contraception has been practiced for over 4000 years and the prevalence of contraceptive use has increased worldwide.the existing methods of contraception are not perfect and their acceptability is limited by side effects and inconvenience.thus there is a real need for new methods of contraception to be developed. the effects of the follicular fluid on the pregnancy rate has b...

BACKGROUND This study aimed to determine amino acid (AA), ammonia and urea concentrations in human ovarian follicular fluid and to compare these concentrations with those in the circulation. METHODS Samples of pre-ovulatory follicular fluid and peripheral venous blood were obtained from 14 IVF patients. High-performance liquid chromatography (HPLC) measurements of 25 AAs were the main outcome...

This study evaluated the levels of c-kit mRNA in goat follicles and the effects of kit ligand (KL) on the in vitro development of cultured preantral follicles. Preantral follicles isolated from goat ovarian cortex were cultured for 18 days in α-MEM(+) supplemented with KL (0, 50 or 100 ng/mL) in the absence or presence of follicle stimulating hormone (FSH). Real-time PCR showed that c-kit mRNA ...

Potential antimicrobial activity of follicular fluid and its selectivity remains open questions in field of human and animal reproduction. In our work we examine antimicrobial activity of porcine follicular fluid on coliform bacteria, as Gram-negative, and B. cereus, as Gram-positive, by incubation of different dilatations of bacteria with follicular fluid in cultivation medium and microbiologi...

Our previous study reported microorganisms in human follicular fluid. The objective of this study was to test human follicular fluid for the presence of microorganisms and to correlate these findings with the in vitro fertilization (IVF) outcomes. In this study, 263 paired follicular fluids and vaginal swabs were collected from women undergoing IVF cycles, with various causes for infertility, a...

BACKGROUND Human follicular fluid contains several substances, such as cytokines and growth factors, which may affect follicular growth and maturation. The present study examines the relative contribution of macrophages and granulosa cells in the production of vascular endothelial growth factor (VEGF) and adrenomedullin in the human ovulatory follicle. METHODS Both follicular fluid samples an...

BACKGROUND A poor quality of oocytes and embryos and a low fertilization rate have been found in polycystic ovary syndrome (PCOS) patients. An inverse association between follicular fluid homocysteine (Hcy) levels and oocyte and embryo quality has also been demonstrated. We examined the relationship between follicular fluid Hcy concentrations and oocyte and embryo quality in PCOS patients under...

background: some studies propose that changes in leptin concentrations (above or under the normal range) result in infertility. therefore, we investigated serum and follicular fluid leptin concentrations in infertile women with polycystic ovary syndrome (pcos). objective: to study serum and follicular fluid leptin concentrations in infertile women with pcos. materials and methods: we conducted ...

Background Polycystic ovary syndrome (PCOS) is the most common endocrine disorder. The roles of vitamin D in the regulation of metabolic modulations specifically involving insulin action and reproduction processing are introduced. In addition, obesity appears to be closely associated with severity of PCOS. The aim of this study was to evaluate the effect of BMI on vitamin D levels in follicular...

OBJECTIVE To determine follicular-fluid neurotrophin levels in women undergoing assisted reproductive techniques for different etiologies of infertility. DESIGN Prospective observational study. SETTING Academically affiliated assisted reproductive techniques unit. PATIENT(S) One hundred six patients undergoing an IVF cycle for different etiologies of infertility. INTERVENTION(S) Assessm...