introduction: the fate of xenobiotics that is present, increasing day by day. the increasing fates altered or inhibit the metabolic activities like detoxification and biotransformation. methods: the present study highlights this slow biotransformation and detoxification on the basis of specific enzymes which have a say in assessment of mercuric chloride toxicity and modulation by panax ginseng ...

glutathione s-transferases (gsts) are a major family of detoxification enzymes which possess a wide range of substrate specificities. interest in insect gsts has primarily focused on their role in insecticide resistance. in this study, following world health organization (who) routine susceptibility test, dna was extracted from specimens of anopheles stephensi collected from the kazeroon distri...

the aim of this study was to detect the glutathione s-transferase(gst) enzyme activity of healthy / cystic liver as a diagnostic biomarker for hydatidosis. in order to compare with liver tissue, the level of the gsts enzyme activity of parasite was also determined.parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with pbs buffer. collected parasi...

The mite Sarcoptes scabiei causes sarcoptic mange, or scabies, a disease that affects both animals and humans worldwide. Our interest in S. scabiei led us to further characterise a glutathione S-transferase. This multifunctional enzyme is a target for vaccine and drug development in several parasitic diseases. The S. scabiei glutathione S-transferase open reading frame reported here is 684 nucl...

OBJECTIVE We investigated the role of the glutathione S-transferase A1, M1, P1 and T1 gene polymorphisms and potential effect modification by occupational exposure to different chemicals in Serbian bladder cancer male patients. PATIENTS AND METHODS A hospital-based case-control study of bladder cancer in men comprised 143 histologically confirmed cases and 114 age-matched male controls. Delet...

A hitherto unknown cytosolic glutathione S-transferase from rat liver was discovered and a method developed for its purification to apparent homogeneity. This enzyme had several properties that distinguished it from other glutathione S-transferases, and it was named glutathione S-transferase X. The purification procedure involved DEAE-cellulose chromatography, (NH4)2SO4 precipitation, affinity ...

We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate.

The chronic effect of environmental methomyl on the antioxidant system in testis Nile tilapia (Oreochromis niloticus) and its recovery pattern was investigated. Tilapia were exposed to sublethal concentrations 0.2, 2, 20 200 ?gL?1 for 30 days thereafter moved methomyl-free water 18 days. Antioxidant levels testis, including glutathione peroxidase, catalase, glutathione-S-transferase, reductase,...