By isolation of a mixed disulfide product of glutathione and cysteine, glutathione peroxidase was shown to be highly specific for only one donor substrate. Using the coupled assay of NADPH and yeast glutatione reductase, which is highly specific for flutathione disulfide, it was shown that the apparent inhibition of glutathione peroxidase by mercaptoethanol can be described kinetically and that...

An optimized coupled test system for glutathione peroxidase in red blood cells is presented. In the improved assay, tert. butyl hydroperoxide was employed as an acceptor substrate at twenty times Km(app) concentration. By aid of a batching procedure for instant separation of hemoglobin from glutathione peroxidase, it could be proved that cyanmethemoglobin in the modified procedure does not affe...

Silicone and Glutathione application are including systemic acquired resistance or induced against plant diseases against. It is a successful means to control rot disease it leads fundamental effects on the physiology of host, as use in agent hostile Fusarium solani causal Broad bean root .The results this study appeared that have make less tot incidence severity plants increase resistance-Link...

BACKGROUND This investigation describes the relationship between glutathione peroxidase activities, an antioxidant enzyme, and the oxidative status in diabetic rat blood. METHODS Malondialdehyde level and glutathione peroxidase activity were measured by spectrophotometric techniques. RESULTS AND CONCLUSIONS Malondialdehyde content in the diabetic rats group was increased compared to that in...

Toshio Kaneko, MD, Third Department of Internal Medicine, Yamaguchi University School of Medicine, Koguchi, Ube Yamaguchi 755 (Japan) Oxidative damage of red cell membrane is known to be accelerated in sickle cell disease. However, the exact mechanism of this acceleration is unclear. We have read with interest the recent article entitled ‘Selenium and Glutathione Peroxidase Levels in Sickle Cel...

the present study aimed to evaluate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (i/r) injury in rats.forty male rats were randomly divided into sham, control (i/r injury) and three crocin-pretreated groups. to induce i/r lesions, the celiac artery was clamped for 30 min and then the clamp was removed to allow reperfusion for 3 h. pretreated-rats rec...

objective: environmental pollution is of major concern today and lead is considered to be one of the most important environmental pollutants. long-term contact with lead causes harmful effects to humans. this study seeks to determine the effects of curcuma longa (turmeric extract) consumption and exercise training on glutathione peroxidase and protein carbonyl in kidney and spleen tissues from ...

background oxidative stress plays a major pathogenic role in liver injury following chronic hepatitis b. glutathione peroxidase (gpx) has a central role in regulating the oxidative state. hepatitis b virus (hbv) results in down-regulation of gpx. on the other hand, iron homeostasis is disrupted in hbv infected patients. therefore, the objective of this study was to assess the interplay of gpx a...

BACKGROUND The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise. METHODS Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes. RESULTS The optimization, when applied to age paired rats, both nulligravid and ...

A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those ...