Bacillus megaterium N.C.T.C. no. 10342 exhibits glutamate synthetase (EC 2.6.1.53) and glutamate dehydrogenase (EC 1.4.1.4) activities. Concentrations of glutamate synthase were high when the bacteria were grown on 3mM-NH4Cl and low when they were grown on 100mM-NH4Cl, whereas glutamate dehydrogenase concentrations were higher when the bacteria were grown on 100mM-NH4Cl than on 3mM-NH4Cl. Gluta...

Pathways of ammonia assimilation into glutamic acid were investigated in ammonia-grown and N2-fixing Clostridium kluyverii and Clostridium butyricum by measuring the specific activities of glutamate dehydrogenase, glutamine synthetase, and glutamate synthase. C. kluyverii had NADPH-glutamate dehydrogenase with a Km of 12.0 mM for NH4+. The glutamate dehydrogenase pathway played an important rol...

The serum levels of the mitochondrial enzymes), aspartate transatninase II, malate dehydrogenase II and glutamate dehydrogenase were studied in 82 cases of acute hepatitis. The activity ratio alanine transaminase/glutamate dehydrogenase and the C/M-ratios of aspartate transaminase and malate dehydrogenase were also studied. The serum levels of the mitochondrial en2ymes glutamate dehydrogenase, ...

Glutamate dehydrogenase becomes density labeled through the incorporation of deuterium and (15)N when detached oat leaves (Avena sativa var. Fulghum) are incubated in the presence of ammonia. The enzyme has been isolated by means of DEAE-cellulose chromatography, ammonium sulfate precipitation, isopycnic equilibrium centrifugation, and disc electrophoresis from leaves fed l-methionine-(35)S. Ra...

In the course of preparing carbamyl phosphate synthetase from frog liver, a crystalline protein fraction was obtained which proved to be glutamate dehydrogenase. Because of the considerable interest in this enzyme and the striking differences in certain properties of this enzyme from different animal sources (l-6), it seemed of importance to study the kinetic properties of crystalline frog live...

We measured the activities of two mitochondrial enzymes, the mitochondrial form of aspartate aminotransferase (EC 2.6.1.1) and glutamate dehydrogenase (EC 1.4.1.2), in the serum of apparently healthy persons (n = 84) and patients suffering from chronic liver diseases (n = 43). The distribution of activities for glutamate dehydrogenase, but not mitochondrial aspartate aminotransferase, was sex-d...

Ammonia is the chief excretory product in fishes. However, non-availability of enough of water in the habitat, may lead to the formation of urea, in fishes. In the present study, the possible role of urea formation to avoid the toxicity of ammonia under water-restricted condition was tested in Channa gachua. Circulatory urea and ammonia were estimated in the blood of the fishes and glu...

Ammonia is the chief excretory product in fishes. However, non-availability of enough of water in the habitat, may lead to the formation of urea, in fishes. In the present study, the possible role of urea formation to avoid the toxicity of ammonia under water-restricted condition was tested in Channa gachua. Circulatory urea and ammonia were estimated in the blood of the fishes and glu...

The active NAD-dependent glutamate dehydrogenase of wild type yeast cells fractionated by DEAE-Sephacel chromatography was inactivated in vitro by the addition of either the cAMP-dependent or cAMP-independent protein kinases obtained from wild type cells. cAMP-dependent inhibition of glutamate dehydrogenase activity was not observed in the crude extract of bcy1 mutant cells which were deficient...