The β-glucosidase from almond was immobilized using different strategies: ionic adsorption on aminated (MANAE)-agarose beads at pH 5, 7 and 9, followed by glutaraldehyde pre-activated supports. of the immobilization altered to allow enzyme molecule orientations support surface. concentration exhibited an effect stability free enzyme. Immobilization ion exchange maintained up 80% activity β-gluc...

The lung provides a portal of entry for drug delivery that could be used to administer anticonvulsant substances to prevent or abort seizures. Here, we demonstrate that intrapulmonary propofol hemisuccinate (PHS) rapidly confers seizure protection in various rodent chemoconvulsant models. Propofol is a powerful anticonvulsant substance at subanesthetic doses, but it is a viscous, water-immiscib...

This study evaluated the anti-biofilm activity of sphingosine, phytosphingosine (PHS), and sphinganine for: (i) anti-adherence activity on hydroxyapatite (HA) surfaces; and (ii) bactericidal activity on different Streptococcus mutans phenotypes (i.e. planktonic cells and cells from a disrupted biofilm). For this, HA discs treated with sphingolipids were incubated with S. mutans and the number o...

the snail caucasotachea lencoranea mousson is a pest of citrus in the north provinces of iran. in order to adopt a better control of the pest and develop optimal management strategies, an understanding of the digestive enzymes’ function is inevitable. snails were collected from some citrus orchards in the northern provinces of the country and digestive carbohydrases including ?-glucosidase, ?-g...

When Escherichia coli was incubated at the growth-refractory temperatures of 48 and 54 degrees C, expression of the cel operon was demonstrated by phospho-beta-glucosidase activity. This enzyme activity was also detected at the growth-refractory temperatures in Salmonella typhimurium and Pseudomonas aeruginosa. Thermotolerant and mesothermophilic mutants of E. coli, S. typhimurium, and P. aerug...

proteases are important enzymes that their role in various industries is undeniable. however, keeping enzymes stable during its activity in harsh conditions is so important. in this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and phs was evaluated. first silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

Use of biosurfactants as a green stabilizer for the biological synthesis of gold nanoparticles (AuNPs) is now emerging as nontoxic and environmentally acceptable "green chemistry" procedures. Stability of AuNPs at different pHs is very important because our body has different pHs. This paper addresses this issue. In this work, first P. aeruginosa PTCC 13401 was used to produce rhamnolipid biosu...

Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

The fungus Penicillium purpurogenum was found to produce intracellular ß-glucosidase. Maximum activity of ß-glucosidase was observed on sucrose. Various cultural parameters of cultivation of P. purpurogenum for production of ß-glucosidase were optimized. Maximum enzyme content was observed after 96 hours of cultivation at 30°C. Addition of amino acids histidine and cysteine induced ß-glucosidas...