Staphylococcus aureus secretes autolysin (Atl) to complete cell division by hydrolyzing its thick cell wall layer at a designated site, known as the equatorial surface ring. Secreted pro-Atl (1256 amino acids) is cleaved at residues 198 and 775 to generate a pro-peptide, amidase and glucosaminidase, respectively. Here we examined the mechanism that directs amidase and glucosaminidase to the cel...

Background: The musculoskeletal repair, in most cases, follows the typical inflammatory process. Although majority of treatments result good prognosis, knowledge concerning basic biochemical principles, regeneration and repair mechanisms skeletal muscles may help recovery accelerate return to sport, helping carefully choose more appropriate therapeutic techniques for injury management. Objectiv...

Feeding sodium deoxycholate orally to rats for four days caused depression of the activity of the small intestinal enzymes lactase, sucrase, maltase, alkaline phosphatase, and N-acetyl-beta-glucosaminidase. The first four are brush border enzymes, the last a lysosomal enzyme. Alkaline phosphatase activity recovered very rapidly and rebounded to above the normal level within 24 hours. The activi...

1. Progression to renal failure may be linked to the degree of proteinuria through tubulo-interstitial mechanisms. However, there are no data in man on the kinetics of proximal renal tubular protein catabolism or markers of tubular injury before and after lisinopril. We developed a method to allow such studies, and found increased tubular catabolism of 99mTc-labelled aprotinin (Trasylol) in pat...

This is a review article regarding the new types of Mucopolysacharidose disease and re­lated diagnostic tests. Recently has been found that Hurler's and Scheie's diseases are due to the deficiency of Alpha-Iduronidase and San­filippo's disease is due to Heparan-N-Sulphatase and N-Acetyl-Alpha-D-Glucosaminidase defici­ency. 

Monocytes from peripheral blood and synovial fluid of patients with definite and classic rheumatoid arthritis spontaneously produced significantly greater amounts of prostaglandin E2 (PGE2), leukotriene B4 (LTB4), and interleukin-1 beta (IL-1 beta) than samples of peripheral blood from normal controls. Peripheral blood monocytes from patients with rheumatoid arthritis produced significantly gre...

from case 2, however, showed a shoulder at pH4.5 due probably to some residual glucosaminidase A and glucosaminidase B activity. The optimum for both glucosaminidase and galactosaminidase activities ofleucocytes from this patient was pH4.5; here a greater proportion of hexosaminidase A and hexosaminidase B remains, masking the C form. The total hexosaminidase activity of these leucocytes was 4 ...

The aim of this study was to establish and quantify changes in the activities of total, free and bound fractions of pancreatic lipase, galactoso-6-sulphatase, beta-D-galactosidase and N-acetyl-beta-D-glucosaminidase in the course of alloxan-induced diabetes mellitus. Rabbits were divided into a control group and groups injected with alloxan on the 21st, 42nd, 90th and the 180th day, after which...

N-acetyl-beta-D-glucosaminidase is a high molecular-weight lysosomal enzyme found in many tissues of the body. It cannot pass into glomerular ultrafiltrate due to its high molecular weight. However, this enzyme shows high activity in renal proximal tubular cells, and leaks into the tubular fluid as the ultrafiltrate passes through proximal tubules. When proximal tubular cells are injured due to...