نتایج جستجو برای: genomic modification
تعداد نتایج: 240732 فیلتر نتایج به سال:
We present a method for cloning restriction-modification (R-M) systems that is based on the use of a lethal plasmid (pKILLER). The plasmid carries a functional gene for a restriction endonuclease having the same DNA specificity as the R-M system of interest. The first step is the standard preparation of a representative, plasmid-borne genomic library. Then this library is transformed with the k...
DNA methylation is related closely to sequence contexts and chromatin modifications; however, their potential differences in different genomic regions across cell types remain largely unexplored. We used publicly available genome-scale DNA methylation and histone modification profiles to study their relationships among different genomic regions in human embryonic stem cells (H1), H1-derived neu...
Primordial germ cells (PGCs), the precursors of germ cells, form during early embryonic development. To date, studies on the PGCs of many species have investigated specification, proliferation, and differentiation, and have created various applications using model animal systems. Avian species are optimal vertebrates in which to examine germ cell development during embryogenesis, because of the...
We compare two techniques which enable selective, nucleotide-specific covalent modification of human genomic DNA, as assayed by quantitative ligation- mediated PCR. In the first, a purine motif triplex-forming oligonucleotide with a terminally appended chlorambucil was shown to label a target guanine residue adjacent to its binding site in 80% efficiency at 0.5 microM. Efficiency was higher in ...
When cloning genomic DNA fragments, the efficiency is greatly reduced as the size of the restriction fragment increases. Libraries derived from the total genome are therefore biased because shorter fragments will be cloned preferentially. When cloning a particular gene, it is most desirable to determine a restriction enzyme that will generate a fragment of a reasonable size for the gene of inte...
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