and rVIV 2 primers, the A-type DNA, respectively. The genus-specifi c DNA amplifi ed both types at random. Some colleagues have experienced similar diffi culties with the primers pmk 8 and pmkr 9 in samples from Vietnam (5); 2 of 5 samples gave false positive results for P. knowlesi. Unfortunately, their report did not mention which species was actually amplifi ed (5). Until recently, we had no...

The use of reproductive and genetic technologies can increase the efficiency of selective breeding programs for aquaculture species. Four technologies are considered, namely: marker-assisted selection, DNA fingerprinting, in-vitro fertilization, and cryopreservation. Marker-assisted selection can result in greater genetic gain, particularly for traits difficult or expensive to measure, than con...

The use of ribosomal RNA (rRNA) as molecular marker to identify microbial populations is now routinely used in microbial ecology. One of the strategies herein is cloning of PCR products obtained from environmental DNA to explore the diversity of microbial communities without the need of cultivation. Although successful, this approach is for practical reasons not suited to study the complex dyna...

fusarium solain is the most important pathogen of huge range of plant hosts, especially potato in the word, which causes tuber rot in storage and root rot of potato plants in fields. fifty four isolates from potato, bean, chickpea and cucurbit (melon, watermelon and cucumber) was subjected in a study through analysis of vegetative compatibility groups (vcgs) and rep-pcr dna fingerprinting. nit ...

The presence of enterobacterial repetitive intergenic consensus (ERIC) sequences was demonstrated for the first time in the genome of Mycobacterium tuberculosis; these sequences have been found in transcribed regions of the chromosomes of gram-negative bacteria. In this study genetic diversity among clinical isolates of M. tuberculosis was determined by PCR with ERIC primers (ERIC-PCR). The stu...

Several genetic loci have been utilized to genotype isolates of Mycobacterium tuberculosis. A shortcoming of the most commonly used method, IS6110 fingerprinting, is that it does not adequately discriminate between isolates having few copies of IS6110. This study was undertaken to compare pTBN12 fingerprinting of polymorphic GC-rich repetitive sequence genes and spoligotyping of the direct repe...

Most previous studies on genetic fingerprinting and cultivar relatedness in sweet cherry were based on isoenzyme, RAPD, and simple sequence repeat (SSR) markers. This study was carried out to assess the utility of single nucleotide polymorphism (SNP) markers generated from 3' untranslated regions (UTR) for genetic fingerprinting in sweet cherry. A total of 114 sweet cherry germplasm representin...

DNA fingerprinting has been hailed by law-enforcement officials as the 20th century’s most important breakthrough in forensic science. They are eager to use the new technology to identify and prosecute violent criminals as well as to exonerate innocent persons who are suspects in criminal cases. Although DNA finger-printing has not yet been adequately tested in the courts, the State Attorney Ge...

Suspended sediments in fluvial systems originate from a myriad of diffuse and point sources, with the relative contribution from each source varying over time and space. The process of sediment fingerprinting focuses on developing methods that enable discrete sediment sources to be identified from a composite sample of suspended material. This review identifies existing methodological steps for...