نتایج جستجو برای: gene products
تعداد نتایج: 1396770 فیلتر نتایج به سال:
background: splicing by overlap extension (soe) pcr is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.objectives: we introduced a nested-soe-pcr (n –soe-pcr) in order to increase the specificity and generating mutations in a gene by soe-pcr. materials and methods: genomic dna from bacillus thermoca...
Background and Objective: Antibody against Pseudomonas aeruginosa exotoxin A can be used in immunotherapy together with antibiotics to treat acute burn patients. Exotoxin A is one of the virulence factors in Pseudomonas aeruginosa that comprises of three domains, binding domain, translocation and catalytic domain. The purpose of this study was to produce recombinant domain of the catalytic part...
Background and aim: Staphylococcus aureus can colonizes variety of body sites such as skin, coetaneous membrane and especially anterior nares. The coagulase gene (coa) typing is a technique in which the organism is typed based on the polymorphic region of the coa gene. The aim of the present study was to determine the coa based typing of S. aureus isolates from health care workers in two teach...
the lpsa gene, a late acting gene in the biosynthetic pathway of ergovaline, a suspected causative agent for fescue toxicosis in cattle, has been cloned from neotyphodium lolii, an endophytic fungus of lolium perenne. in this study, a similar gene was detected in several strains of endophytic neotyphodium spp. isolated from grass hosts endogenous to iran using direct and nested-pcr assays. exce...
Background and purpose: Giardia and Blastocystis are common parasites of humans with a wide range of hosts. The aim of this study was to identify the genotypes of Giardia intestinalis and Blastocystis hominis in individuals attending Bavi health centers in Khuzestan province, Iran. Materials and methods: In this study, 30 positive stool samples of Giardia and Blastocystis were collected from i...
ABSTRACT Background and Objectives: Determining the genetic relationship between S. aureus isolates is important for epidemiological surveillance and control of infections caused by this bacterium. The present study was conducted to determine polymorphisms of coagulase gene (coa) among S. aureus isolates from pastry and cheese samples usi...
The lpsA gene, a late acting gene in the biosynthetic pathway of ergovaline, a suspected causative agent for fescue toxicosis in cattle, has been cloned from Neotyphodium lolii, an endophytic fungus of Lolium perenne. In this study, a similar gene was detected in several strains of endophytic Neotyphodium spp. isolated from grass hosts endogenous to Iran using direct and nested-PCR assays. Exce...
مطالعات انجام شده روی مکانیسمهای تکوینی و تکاملی گلبولهای قرمز منجر به دستیابی بشر به مفاهیم پایه و مهمی در ارتباط با مکانیسمهای عمومی تنظیم بیان ژن وشکل گیری بافتها شده است. تمایز اختصاصی به رده ارتیروئید و هر رده دیگری، شدیداً وابسته به تنظیم در سطح بیان ژن و فاکتورهای کنترلی خاص نظیر سیتوکین ها، فاکتورهای نسخه برداری ویژه، عناصر کنترل کننده چرخه سلولی، تکثیر،آپوپتوز و عناصر سیگنالینگ داخل سلو...
This study reports on the molecular identification of fish species from processed products which had a priori been classified as belonging to 5 important species in Iran for human consumption. DNA barcoding using direct sequencing of an approximately 650bp of mitochondrial Cytochrome oxidase subunit I (COI) gene revealed incorrect labeling of Narrow-barred Spanish mackerel samples. High occurre...
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