The Ras(17N) dominant negative antagonizes endogenous Ras function by forming stable, inactive complexes with Ras guanine nucleotide exchange factors (GEFs; e.g., SOS1). We have used the growth-inhibitory phenotype of Ras(17N) to characterize two aspects of Ras interaction with GEFs. First, we used a nonprenylated version of Ras(17N), designated Ras(17N/186S), which no longer associates with th...

Feature selection for filtering HTTP-traffic in Web application firewalls (WAFs) is an important task. We focus on the GenericFeature-Selection (GeFS) measure [4], which was successfully tested on low-level package filters, i.e., the KDD CUP’99 dataset. However, the performance of the GeFS measure in analyzing high-level HTTP-traffic is still unknown. In this paper we study the GeFS measure for...

Guanine nucleotide exchange factors (GEFs) control the site and extent of GTPase activity. Longin domains (LDs) are found in many Rab-GEFs, including DENNs, MON1/CCZ1, BLOC-3 and the TRAPP complex. Other GEFs, including Ragulator, contain roadblock domains (RDs), the structure of which is closely related to LDs. Other GTPase regulators, including mglB, SRX and Rags, use LDs or RDs as platforms ...

What is a GEF? GEF stands for guanine-nucleotide exchange factor, any of a large and diverse group of proteins that catalyze the release of GDPand the subsequent uptake of GTPby members of the Ras and heterotrimeric G-protein superfamilies (referred to here collectively as G proteins). When bound to GTP, G proteins are in the active conformation. Upon hydrolysis of this bound GTP, by virtue of ...

Efficient collective migration depends on a balance between contractility and cytoskeletal rearrangements, adhesion, and mechanical cell-cell communication, all controlled by GTPases of the RHO family. By comprehensive screening of guanine nucleotide exchange factors (GEFs) in human bronchial epithelial cell monolayers, we identified GEFs that are required for collective migration at large, suc...

Accurate forecasting of wind speed holds significant importance for the economic and social development humanity. However, existing numerical weather predictions have certain inaccuracies due to various reasons. Therefore, it is highly necessary perform statistical post-processing on forecasted results. traditional linear methods possess inherent limitations. Hence, in this study, we employed t...

Genetic epilepsy with febrile seizures plus (GEFS+) is characterized by childhood-onset epilepsy syndrome. It involves febrile seizures and a variety of afebrile epileptic seizure types within the same pedigree with autosomal-dominant inheritance. Approximately 10% of individuals with GEFS+ harbor SCN1A, a gene mutation in one of the voltage-gated sodium channel subunits. Considerably less comm...

In 1978, Charlotte Dravet described thècryptogenic' epilepsy syndrome severe myoclonic epilepsy of infancy (SMEI) (Dravet, 1978). This severe generalized epileptic encephalopathy begins at around 6 months of age with febrile hemiclonic or generalized status epilepticus. Hemiclonic status typically recurs involving each side independently. After 1 year of age, other seizure types appear includin...