The control of gastric pepsin secretion continues to excite controversy. The diversity in the results obtained by different investigators may be due, at least in part, to the varying techniques employed to estimate pepsin activity. Thus, edestin (Polland and Bloomfield, 1929), gelatin (Gilman and Cowgill, 1930), egg albumen (Vineberg and Babkin, 1931), beef haemoglobin (Anson and Mirsky, 1932),...

Few articles on concentration of pepsin in the gastric juice of patients with disease of the stomach and duodenum are to be found in recent literature, and the results reported by different observers are not always in accord. We shall consider here only the concentration of pepsin in the gastric juice of persons suffering with the syndrome of peptic ulcer. Wilcox (5), in 1908, reported that the...

The irreversible disappearance of the activity of pepsin in alkaline solution was first recorded by Kfihne (1). I t was confirmed by Langley (2), who observed also " tha t the destruction by sodium carbonate solutions goes on very much more slowly at 15°C. than at body temperature." Langley's experiments were carried out with neutralized filtrates of extracts of rabbit gastric mucosa made with ...

To investigate the mechanism of the anti-peptic action of ecabet sodium (TA-2711) observed in pylorous-ligated rats, effects of this drug on the peptic activity of rat gastric juice, purified hog pepsin and pepsinogen were studied in vitro. After incubation with or without ecabet at acidic pH, the reaction mixture was centrifuged, and the peptic activity of the supernatant was measured. Ecabet ...

OBJECTIVES/HYPOTHESIS To assess if, as previously reported in the literature, bile acids inhibit pepsin activity, resulting in pepsin having a less important role in laryngopharyngeal damage in reflux disease. STUDY DESIGN Prospective translational research study. METHODS A total of 78 patient's fasting gastric juice samples were obtained from routine endoscopy. The total bile acid (TBA) co...

A previous paper (2) described the isolation and crystallization of gastricsin from human gastric juice and the physical, chemical, and enzymatic properties of gastricsin as compared to human pepsin. It is the purpose of the present report to describe the purification and properties of a zymogen from human gastric mucosa. The activation of this zymogen produces both gastricsin and human pepsin.

Acid gastro-oesophageal reflux (GOR) and gastric aspiration have been labelled as risk factors for chronic rejection bronchiolitis obliterans syndrome (BOS) after lung transplantation (LTx). The present study aimed to further characterise GOR (both acid and nonacid) and the degree of gastric aspiration in LTx recipients both with and without BOS. Impedance-pH recordings were used for GOR detect...

The ingestion of nucleic acids (NAs) as a nutritional supplement or in genetically modified food has attracted the attention of researchers in recent years. Discussions over the fate of NAs led us to study their digestion in the stomach. Interestingly, we found that NAs are digested efficiently by human gastric juice. By performing digests with commercial, recombinant and mutant pepsin, a prote...

Using a radioimmunoassay directed toward the COOH-terminal, biologically active region of mammalian neurotensin (NT), the rapid (within seconds) generation of immunoreactive NT (iNT) during acid extraction of mammalian and avian gastric tissues has been demonstrated. Levels of iNT were shown to increase 25-200-fold in time. The reaction occurred in 0.1 N HCl and 2 N acetic acid and was prevente...

Sonic hedgehog (Shh) is not only essential to the development of the gastrointestinal tract, but is also necessary to maintain the characteristic acid-secreting phenotype of the adult stomach. Gastrin is the only hormone capable of stimulating gastric acid and is thus required to maintain functional parietal cells. We have shown previously that gastrin-null mice display gastric atrophy and meta...