The detection of galactomannan (GM) in the serum of immunocompromised patients is widely used for the early diagnosis of invasive aspergillosis (6). The test may also be useful when applied to bronchoalveolar lavage (BAL) fluid specimens for clinical diagnosis (5), though not FDA approved for this use. One important limiting factor for GM testing is the potential for false-positive results. Mos...

This report describes the use of the polymerase chain reaction (PCR) and galactomannan detection to detect aspergillus in the continuous ambulatory peritoneal dialysis (CAPD) fluid and blood of a patient with multiple myeloma on CAPD and immunosuppressive treatment. Diagnosis of aspergillosis was initially made by conventional culture of CAPD fluid, but the PCR and galactomannan assays also det...

Introduction and Aim: Fenugreek (Trigonella foenum graceum) is extensively cultivated in several Asian nations. The leaves seeds of this plant are well recognized for their potential against diabetes, some cancers imparting immunity. They often employed as adhesives emulsifying agents. Polysaccharides can be found abundance fenugreek seeds. Galactomannan, a water-soluble polysaccharide, efficie...

We show that galactomannan, a polysaccharide consisting of a mannose backbone with galactose side groups present on the cell wall of several fungi, induces a reprogramming of the inflammatory response in human macrophages through dectin-1 receptor. The nuclear factor kappa-light-chain-enhancer of activated B cells 2 (NFκB2)/p100 was overexpressed after galactomannan challenge. Knocking down NFκ...

Galactomannan extracted from seeds of Cassia grandis with 0.1M NaCl, followed by ethanol precipitation, presented a yield of 36 ± 8%. The polysaccharide has a constant mannose/galactose ratio (2.44:1). Methylation analysis, one and two dimensional NMR spectroscopy confirmed that the polysaccharide has a central core composed of 4-linked β-mannose units, with branches of galactose, linked to the...

introduction: invasive aspergillosis is a major cause of morbidity and mortality in hematologic malignant patients which have received intensive cytotoxic therapy or undergone blood and marrow transplantation (bmt). early clinical and radiological diagnosis of ia is almost impossible and so mortality is very high. the main purpose of our study was to assay the diagnostic value of serum galactom...

We compared the usefulness of a polymerase chain reaction (PCR) assay for the early diagnosis of invasive pulmonary aspergillosis with the serodiagnosis of sufficient concentrations of galactomannan using the same serum samples. A patient was treated with prednisolone for the management of hepatitis. Computed tomography (CT) scan of the chest showed the nodular shadow with a cavity containing a...

It is well known that cross reactions with other fungal pathogens including Histoplasma capsulatum can occur with the use of the Platelia™ Aspergillus galactomannan assay. We report two patients with confirmed blastomycosis whose bronchoalveolar lavage (BAL) fluid tested positive for Aspergillus galactomannan despite no evidence of aspergillosis.

Aging is caused by free radicals, one of which ultraviolet light can form ROS. Galactomannan compounds have the potential as antioxidants that counteract radicals. Kolang kaling contains many galactomannan compounds. processed into a peel-off gel mask, where PVA and glycerin are essential ingredients. polymer film layer, but if layer not balanced, used humectant. The results FTIR analysis showe...

Galactomannan biosynthesis in legume seed endosperms involves two Golgi membrane-bound glycosyltransferases, mannan synthase and galactomannan galactosyltransferase (GMGT). GMGT specificity is an important factor regulating the distribution and amount of (1-->6)-alpha-galactose (Gal) substitution of the (1-->4)-beta-linked mannan backbone. The model legume Lotus japonicus is shown now to have e...