DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

with advent and development of dna recombinant technology and advantages of p. pastoris expression system, fusion (f) protein of pprv expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against ppr disease. in this study, f gene of pprv nigeria 75/1 strain (1637 bp) was amplified using rt-pcr and purified. it was then clon...

The herpes simplex virus type 1 (HSV-1) tegument protein, VP22 has been reported to have the property of intercellular transport. The previous studies have shown that following expression of a fusion protein containing VP22 it spreads to every cell in a monolayer and concentrates in the nucleus. In spite of these reports, some studies have shown that VP22 trafficking and its nucleus accumulatio...

dna coding for the core antigen from hepatitis b virus (hbcag) was amplified, cloned and propagated in e. coli. the core protein was expressed in e. coli and the product was readily detected by western blot. this protein can be used as a diagnostic material in serum screening tests. to increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

background: dense granule antigens (gra antigens) of toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. previous studies reported expression of amino terminal gra8 protein in fusion with large tags such as glutathione-s-transferase. the present study aimed to produce soluble full length immunogenic gra8 in bacteria. methods: gra8 comple...

Background: Targeting of CD20 antigen with monoclonal antibodies has become the mainstay in the treatment of non-Hodgkin's lymphomas and immunotherapeutic depletion of malignant B cells. Accessibility of antigen is one of the crucial factors in development of monoclonal antibodies against this antigen. One major problem in expression of full length CD20 is aggregation and misfolding. Therefore,...

Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...

background and aims: ndv (newcastle disease virus) is one of the viruses that cause disease in avian with severe economic losses in the poultry industry in many countries. fusion protein (f) which plays a major role in the virus pathogenicity contains several regions that have a role in the fusion process. mutation in the sequence of hr1 & hr2 regions of this protein prevents fusion of the viru...

the herpes simplex virus type 1 (hsv-1) tegument protein, vp22 has been reported to have the property of intercellular transport. the previous studies have shown that following expression of a fusion protein containing vp22 it spreads to every cell in a monolayer and concentrates in the nucleus. in spite of these reports, some studies have shown that vp22 trafficking and its nucleus accumulatio...