Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool towards analysing diffusion processes within living biological cells. A model is presented that aims to provide a rigorous theoretical framework from which binding information of proteins from FRAP data can be extracted. A single binding reaction is considered and a set of mathematical equations is introduced...

enhanced oxidative stress in diabetes mellitus may contribute to the pathogenesis of diaetic complications. in this article, the stress oxidative generation has been studied in experimental diabetes by ferric reducing ability of plasma assay, a sensitive and simple method, and by other oxidative damage markers. the ferric reducing ability of plasma values as a total antioxidant capacity were si...

A procedure for a systematic evaluation of fluorescence recovery after photo-bleaching (FRAP) data is presented that allows one to determine distributions of diffusion coefficients. The method provides a straightforward and calibration-free way to quantify multiple diffusion processes when FRAP is measured as a function of time and space by means of confocal laser scanning microscopy. It is ver...

The analogous techniques of photoactivation of fluorescence (PAF) and fluorescence recovery after photobleaching (FRAP) have been applied previously to the study of actin dynamics in living cells. Traditionally, separate experiments estimate the mobility of actin monomer or the lifetime of actin filaments. A mathematical description of the dynamics of the actin cytoskeleton, however, predicts t...

BACKGROUND Epidemiologic evidence on the association of individual antioxidant vitamins and cognition is inconsistent. OBJECTIVE We evaluated the total antioxidant capacity of diets on the basis of the ferric-reducing antioxidant power (FRAP) assay in relation to cognition in older women. DESIGN Starting in 1995, we used a telephone-based cognitive assessment to evaluate cognitive function ...

BACKGROUND Macromolecule mobility is often quantified with Fluorescence Recovery After Photobleaching (FRAP). Throughout literature a wide range of diffusion coefficients for GFP in the cytoplasm of Escherichia coli (3 to 14 µm²/s) is reported using FRAP-based approaches. In this study, we have evaluated two of these methods: pulsed-FRAP and "conventional"-FRAP. PRINCIPAL FINDINGS To address ...

PURPOSE The comparison of nutrition of children with high level of physical activity in dependence on antioxidative efficiency expressed in Ferric Reducing Ability of Plasma--FRAP adapted for saliva. MATERIAL AND METHODS The group consisted of 74 pupils (43 boys and 31 girls) from swimming classes of Sport Championship School in Kraków. FRAP was measured in saliva with the use of colorimetric...

The FRAP-p70 signaling pathway was found to be constitutively phosphorylated/active in MiaPaCa-2 and Panc-1 human pancreatic cancer cells and a pancreatic cancer tissue sample as judged by the retarded electrophoretic mobility of the two major FRAP downstream targets, p70 and 4E-BP1. Treatment of cells with rapamycin, a selective FRAP inhibitor, inhibited basal p70 kinase activity and induced d...

Confocal fluorescence recovery after photobleaching (FRAP) is today the prevalent tool when studying the diffusional and kinetic properties of proteins in living cells. Obtaining quantitative data for diffusion coefficients via FRAP, however, is challenged by the fact that both bleaching and scanning take a finite time. Starting from an experimental case, it is shown by means of computer simula...