A procedure for a systematic evaluation of fluorescence recovery after photo-bleaching (FRAP) data is presented that allows one to determine distributions of diffusion coefficients. The method provides a straightforward and calibration-free way to quantify multiple diffusion processes when FRAP is measured as a function of time and space by means of confocal laser scanning microscopy. It is ver...

The analogous techniques of photoactivation of fluorescence (PAF) and fluorescence recovery after photobleaching (FRAP) have been applied previously to the study of actin dynamics in living cells. Traditionally, separate experiments estimate the mobility of actin monomer or the lifetime of actin filaments. A mathematical description of the dynamics of the actin cytoskeleton, however, predicts t...

BACKGROUND Epidemiologic evidence on the association of individual antioxidant vitamins and cognition is inconsistent. OBJECTIVE We evaluated the total antioxidant capacity of diets on the basis of the ferric-reducing antioxidant power (FRAP) assay in relation to cognition in older women. DESIGN Starting in 1995, we used a telephone-based cognitive assessment to evaluate cognitive function ...

BACKGROUND Macromolecule mobility is often quantified with Fluorescence Recovery After Photobleaching (FRAP). Throughout literature a wide range of diffusion coefficients for GFP in the cytoplasm of Escherichia coli (3 to 14 µm²/s) is reported using FRAP-based approaches. In this study, we have evaluated two of these methods: pulsed-FRAP and "conventional"-FRAP. PRINCIPAL FINDINGS To address ...

PURPOSE The comparison of nutrition of children with high level of physical activity in dependence on antioxidative efficiency expressed in Ferric Reducing Ability of Plasma--FRAP adapted for saliva. MATERIAL AND METHODS The group consisted of 74 pupils (43 boys and 31 girls) from swimming classes of Sport Championship School in Kraków. FRAP was measured in saliva with the use of colorimetric...

The FRAP-p70 signaling pathway was found to be constitutively phosphorylated/active in MiaPaCa-2 and Panc-1 human pancreatic cancer cells and a pancreatic cancer tissue sample as judged by the retarded electrophoretic mobility of the two major FRAP downstream targets, p70 and 4E-BP1. Treatment of cells with rapamycin, a selective FRAP inhibitor, inhibited basal p70 kinase activity and induced d...

Confocal fluorescence recovery after photobleaching (FRAP) is today the prevalent tool when studying the diffusional and kinetic properties of proteins in living cells. Obtaining quantitative data for diffusion coefficients via FRAP, however, is challenged by the fact that both bleaching and scanning take a finite time. Starting from an experimental case, it is shown by means of computer simula...

The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). Binding of the 4E-BPs to eIF4E is regulated by phosphorylation: Hypophosphorylate...

We present FRAPpuccino (or FRAP), a provenancebased fault detection mechanism for Platform as a Service (PaaS) users, who run many instances of an application on a large cluster of machines. FRAP models, records, and analyzes the behavior of an application and its impact on the system as a directed acyclic provenance graph. It assumes that most instances behave normally and uses their behavior ...