One major method used to evaluate the biocompatibility of porous tissue engineering scaffolding materials is MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The MTT cell viability assay is based on the absorbance of the dissolved MTT formazan crystals formed in living cells, which is proportional to the number of viable cells. Due to the strong dye sorption capability ...

The hydrogen acceptor 3-(4,5-dimethylthiazol-2-yl)-2,5 dipheny Itetrazolium bromide (Mil) is commonly utilized to estimate cellular viability in drug screening protocols. The present investigation was prompted, in part, by observations that reduction of MTT to its colored reaction product, MTT formazan, varied between cell lines and with culture age. A correlation was established between the D-...

Soluble formazan assays are widely used for cell number assessment. However, in our hands, we observed frequent occasions in which the actual cell number was at odds with the assay reading. In this study, we have determined that (i) a large proportion of the reading obtained in commonly used culture media can be caused by media component amplification of formazan production in a way that cannot...

In one of the current histochemical methods for dehydrogenases and diaphorases the final product is a metal-formazan dye derived from reduction of an N-thiazolyl-substituted tetrazolium. Sites of enzymic activity consistently appear as intramitochondrial dots 0.2 to 0.3 micro in diameter. When applied to active particles from disrupted mitochondria (Keilin-Hartree preparation, electron transpor...

ÖZ E T Bu çalışmada tetrazolyum tuzları ve formazanların yapıları tanımlanmıştır. Ayrıca formazan-tetrazolyum sistemlerinin önemi ve kullanım alanları açıklanmıştır. Formazanların sahip oldukları conjugate p-system kaynaklanan tautomerleşme özellikleri ve geometrik izomerleri incelenmiştir. Bu bileşiklerin en yaygın kullanılan sentezleri ve yapılarının açıklanmasında önemli olan UV-visible, IR ...

T, . etrazolium stains permit the histochemical demonstration of dehydrogenase enzymes in tissue sections. These stains are soluble compounds which act as hydrogen acceptors developing insoluble formazan precipitates in the reduced state. Blue tetrazolium was first used by DeBerardinis to study frozen corneal tissue sections and subsequently has been used by Kuwabara and Cogan, who studied the ...