Fish eosinophilic granular cells are found in the connective tissues of structures associated with external environment. These similar to mammalian mast terms structural and functional characteristics. The cytoplasmic granules these give different staining reactions depending on fixative type. This study aimed determine properties densities Oscar fish (Astronotus ocellatus Agassiz, 1831) intest...

Clean microscopic slides are dipped in a solution of mercuric chloride and sucrose in 30% alcohol and allowed to dry. A thin transparent film of cytological fixative is thus formed on both sides of the slide. Fixative slides, prepared in this manner, can be stored indefinitely provided that they are not exposed to a warm, humid atmosphere. When a drop of fluid containing bacteria, protozoa, or ...

Over the years, many ideas have been handed down from one technologist to another about what to do and what not to do when fixing cells and tissues. This set of experiments takes into account certain conditions involving the age of the prepared primary fixative, length of time in primary fixative, length of time en bloc stain is on tissue cultured cells and the effect of washing the cells befor...

AIMS The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10% neutral buffered formalin. METHODS Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10% neutral buffered formalin. RESULTS After a short ...

Osmium tetroxide fixation of tissue blocks, as usually effected, is preceded by an acidification of the tissue. This acidification is probably responsible for morphological alterations which are notably disturbing in electron microscopy. The acidification and the resulting morphological alterations cannot be prevented by homogenizing the tissue directly in OsO(4) solutions or by adding enzyme i...