Most current models for replication past damaged lesions envisage that translesion synthesis occurs at the replication fork. However older models suggested that gaps were left opposite lesions to allow the replication fork to proceed, and these gaps were subsequently sealed behind the replication fork. Two recent articles lend support to the idea that bypass of the damage occurs behind the fork...

Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. Replication fork barriers are a commonly encountered problem, which can cause fork collapse and act as hotspots for replication termination. Collapsed forks can be rescued by homologous recombination, which restarts replication. However, ...

Initiation of DNA replication is tightly controlled during the cell cycle to maintain genome integrity. In order to directly study this control we have previously established a cell-free system from human cells that initiates semi-conservative DNA replication. Template nuclei are isolated from cells synchronized in late G1 phase by mimosine. We have now used DNA combing to investigate initiatio...

Fork-join queueing networks model a network of parallel servers in which an arriving job splits into a number of subtasks that are serviced in parallel. Fork-join queues can be used to model disk arrays. A response time approximation of the fork-join queue is presented that attempts to comply with the additional constraints of modelling a disk array. This approximation is compared with existing...

Tuning fork experiments at the undergraduate level usually only demonstrate a tuning fork's linear resonance. In this paper, we introduce an experiment that can be used to measure nonlinear frequency response curve of regular fork. Using double-grating Doppler interferometry, achieve measurement precision oscillation amplitude in range microns. With experimental setup, observe behaviors fork, s...

The progress of replication forks is often threatened in vivo, both by DNA damage and by proteins bound to the template. Blocked forks must somehow be restarted, and the original blockage cleared, in order to complete genome duplication, implying that blocked fork processing may be critical for genome stability. One possible pathway that might allow processing and restart of blocked forks, repl...

Fork-heuristics play a key role in software Thread-Level Speculation (TLS). Current fork-heuristics either lack real parallel execution environment information to accurately evaluate fork points and/or focus on hardware-TLS implementation which cannot be directly applied to software TLS. This paper proposes adaptive fork-heuristics as well as a feedback-based selection technique to overcome the...

Human helicase-like transcription factor (HLTF) exhibits ubiquitin ligase activity for proliferating cell nuclear antigen (PCNA) polyubiquitylation as well as double-stranded DNA translocase activity for remodeling stalled replication fork by fork reversal, which can support damage bypass by template switching. However, a stalled replication fork is surrounded by various DNA-binding proteins wh...

Initially discovered in Escherichia coli, RuvAB proteins are ubiquitous in bacteria and play a dual role as molecular motor proteins responsible for branch migration of the Holliday junction(s) and reversal of stalled replication forks. Despite mounting genetic evidence for a crucial role of RuvA and RuvB proteins in reversal of stalled replication forks, the mechanistic aspects of this process...