نتایج جستجو برای: fmdv

تعداد نتایج: 872  

Journal: :archives of razi institute 2016
h. mahravani m. azimi a. ahmadi f. jeirani

during 2010-2011, real-time pcr procedure was used to detecting fmdv rna on 147 epithelium samples from the field. in this survey, for real-time pcr from 3d gene segment as conserve region selected for tracking all of seven serotypes fmdv. the assay detected the viral rna in all serotypes of fmdv. the rrt-pcr specifically detected fmd virus in sample with greater sensitivity than our convention...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2012
Chrisostom Ayebazibwe Frank Norbert Mwiine Sheila Nina Balinda Kirsten Tjørnehøj Soren Alexandersen

Diagnosis and control of Foot-and-mouth disease virus (FMDV) requires rapid and sensitive diagnostic tests. Two antibody enzyme-linked immunosorbent assay (ELISA) kits, Ceditest® FMDV-NS for the detection of antibodies against the nonstructural proteins of all FMDV serotypes and Ceditest® FMDV type O for the detection of antibodies against serotype O, were evaluated under African endemic condit...

Journal: :Veterinary microbiology 2004
Sandra F Amass Peter W Mason Juan M Pacheco Cheryl A Miller Antonio Ramirez L Kirk Clark Darryl Ragland Jessica L Schneider Simon J Kenyon

The aim of this study was to determine personal hygiene protocols and animal avoidance periods needed to prevent transmission of FMDV (O/TAW/97). Forty-six, 9-week-old barrows free of FMDV were randomly allocated to five treatment groups and a control group. Investigators contacted and sampled FMDV-inoculated pigs for approximately 40 min and then contacted and sampled sentinel pigs after using...

2015
XIAOXI ZHANG HAIXUE ZHENG MINJUN XU YU ZHOU XIANGPING LI FAN YANG QINGYOU LIU DESHUN SHI

Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven‑hoofed animals, which leads to serious economical losses. FMDV is not adequately controlled by vaccination or biosecurity measures. To generate genetically modified FMDV‑resistant animals, a combinatorial expression cassette producing three short hairpin (sh)RNAs was constructed using the lentivirus (LV) vector, LV...

Journal: :The Onderstepoort journal of veterinary research 2014
Mathias Mkama Christopher J Kasanga Raphael Sallu Ezekia Ranga Mmeta Yongolo Misheck Mulumba Mark Rweyemamu Philemon Wambura

Foot-and-mouth disease (FMD) is caused by a virus of the genus Aphthorvirus of the family Picornaviridae. There is great scientific need for determining the transmission dynamics of FMD virus (FMDV) by drawing more attention to the livestock-wildlife interface areas. A variety of literature suggests that buffalo could serve as reservoir of FMDV in wildlife and cattle. However, many FMDV researc...

2016
Moses Tefula Dhikusooka Chrisostom Ayebazibwe Alice Namatovu Graham J. Belsham Hans Redlef Siegismund Sabenzia Nabalayo Wekesa Sheila Nina Balinda Vincent B. Muwanika Kirsten Tjørnehøj

BACKGROUND Foot-and-mouth disease (FMD) is endemic in Uganda in spite of the control measures used. Various aspects of the maintenance and circulation of FMD viruses (FMDV) in Uganda are not well understood; these include the role of the African buffalo (Syncerus caffer) as a reservoir for FMDV. To better understand the epidemiology of FMD at the livestock-wildlife-interface, samples were colle...

2016
Carolina Stenfeldt Michael Eschbaumer Steven I Rekant Juan M Pacheco George R Smoliga Ethan J Hartwig Luis L Rodriguez Jonathan Arzt

UNLABELLED The pathogenesis of persistent foot-and-mouth disease virus (FMDV) infection was investigated in 46 cattle that were either naive or had been vaccinated using a recombinant, adenovirus-vectored vaccine 2 weeks before challenge. The prevalence of FMDV persistence was similar in both groups (62% in vaccinated cattle, 67% in nonvaccinated cattle), despite vaccinated cattle having been p...

2014
Yuan-xing Gu Zong-liang Gao Jian-hua Zhou Jie Zhang Yong-sheng Liu

RNA interference (RNAi) has been proved to be a powerful tool for foot-and-mouth disease virus FMDV inhibition in vitro and in vivo. We established five stable baby hamster kidney 21 cell lines (BHK-21) containing five short hairpin RNAs (shRNAs) expression plasmids (p3D1shRNA, p3D2shRNA, p3D3shRNA, p3D4shRNA, and p3D5shRNA) targeting 3D gene of FMDV. Immunofluorescent assay, virus titration, a...

2011
Teresa Rodríguez-Calvo Fayna Díaz-San Segundo Marta Sanz-Ramos Noemí Sevilla

Foot-and-mouth disease virus (FMVD), one of the most contagious viruses of cloven-hoofed animals, may cause a prolonged, asymptomatic but persistent infection in ruminants, named the "carrier state". However, it remains an open question whether this carrier state occurs in pigs. Here we present quantitative analyses of the duration of FMDV RNA and infectivity in lymphoid and epithelial tissues ...

Journal: :Scientific reports 2016
Dan Li Caoqi Lei Zhisheng Xu Fan Yang Huanan Liu Zixiang Zhu Shu Li Xiangtao Liu Hongbing Shu Haixue Zheng

Foot-and-mouth disease virus (FMDV) is the etiological agent of FMD, which affects cloven-hoofed animals. The pathophysiology of FMDV has not been fully understood and the evasion of host innate immune system is still unclear. Here, the FMDV non-structural protein 3A was identified as a negative regulator of virus-triggered IFN-β signaling pathway. Overexpression of the FMDV 3A inhibited Sendai...

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