Whole Leaves, Photosynthesis, Herbicide Penetration and Detoxification, Fluorometric M ethod The effect of herbicides that inhibit the photosynthetic electron transport at the photosystem II acceptor side has been analyzed in whole plants by using a fluorometric method. The data reported indicate that the apparent variable fluorescence o f the induction curve normalized to the control value pro...

1. Mattingly, D., A simple fluorometric method for the estimation of free il-hydroxycorticoids in human plasma. J. Clin. Pathol. 15,374(1962). 2. Sweat, M. L., Sulfuric acid induced fluorescence of corticosteroids. Anal. Chem. 26,773(1954). 3. Rubin, M., Fluorometry and phosphorimetry in clinical chemistry. Advan. Gun. Cltem. 13, 163 (1970). 4. Van de Vies, J., Individual determination of corti...

For fluorometry of selenium in human blood, hair, and liver and in leaves, we wet-ashed the samples with conventional nitric and perchloric acids, and then extracted piazselenol (complex of Se and 2,3-diaminonaphthalene) in cyclohexane. Selenium was back-extracted from the cyclohexane into nitric acid to remove the fluorometric interferences of trace amounts of organic compounds. This fluoromet...

The fluorometric neuraminidase assay method using 4-methylumbelliferyl-N-Ac-aD-neuraminide as a substrate was investigated for the purpose of ascertaining its validity as a procedure for influenza viral neuraminidase antibody inhibition test. The enzyme antibody inhibition titer obtained by the fluorometric assay system was far weaker than that obtained by the standard colorimetric neuraminidas...

in the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (ldcf) to fluorescent dichlorofluoroscein (dcf) was used for detection of antioxidant properties of plant extracts. the rate of the reaction was followed by monitoring the formation of dcf as a function of time. the antioxidant assay was carried out for different concentrations of gallic acid, salvia mirzayanii...

BACKGROUND AND OBJECTIVES Numerous procedures in biology and medicine require the counting of cells. Direct enumeration of Colony Forming Units (CFUs) is time-consuming and dreary accurate cell counting on plates with high numbers of CFUs is error prone. In this study we report a new indirect cell counting method that was developed based on the use of Redsafe fluorometric assay. The usefulness ...

We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct determination of protoporphyrin in the same samples. In the direct assay the f...