Cortical spreading depression (CSD) is an important disease model for migraine and cerebral ischemia. In this study, we exploit the high temporal and spatial resolution of optical imaging to characterize perfusion-dependent and -independent changes in response to CSD and to investigate the etiology of reflectance changes during CSD. In this experiment, we characterized the optical response to C...

Acanthamoeba keratitis is a serious pathogenic corneal disease, with challenging diagnosis. Standard diagnostic methods include corneal biopsy (involving cell culture) and in vivo reflection corneal microscopy (in which the visualization of the pathogen is challenged by the presence of multiple reflectance corneal structures). We present a new imaging method based on fluorescence sectioned micr...

We propose a technique for efficient storage and importance sampling of fluorescent spectral data. Fluorescence is fully described by re-radiation matrix, which given input wavelength indicates how much energy re-emitted at other wavelengths. However, such representation has considerable memory footprint. To significantly reduce requirements, we the use Gaussian mixture models matrices. Instead...

Optical label-free biosensors have demonstrated advantages over fluorescent-based detection methods by allowing accurate quantification while also being capable of measuring dynamic bimolecular interactions. A simple, high-throughput, solid-phase, and label-free technique, interferometric reflectance imaging sensor (IRIS), can quantify the mass density of DNA with pg/mm(2) sensitivity by measur...

Inflammatory responses after myocardial infarction profoundly impact tissue repair. Yet, efficient tools to serially and noninvasively assess cellular and molecular functions in postinfarct inflammation are lacking. Here we use multichannel fluorescent molecular tomography (FMT) for spatiotemporal resolution of phagocytic and proteolytic activities mediated by macrophages and neutrophils in mur...

According to Gruml luminescence is "the phenomenon of the emission by matter of electromagnetic radiation that for certain wavelengths or restricted regions of the spectrum is in excess of that due to the thermal radiation fiom the material at the same temperature." We encounter several different types of luminescence in everyday life such as fluorescence, phbsphorence, cathodoluminescence and ...

The average velocity of water flow has been simultaneously measured with fluorescent and speckle imaging methods. The measured velocities with two methods are in good agreement with each other and it confirms that the speckle imaging method can be used as a confident method to measure the velocity of water flow in a dry leaf. Also the velocity of water flow through thick and thin xylems of a le...

High-resolution cerebral vasculature imaging has applications ranging from intraoperative procedures to basic neuroscience research. Laser speckle, with spatial contrast processing, has recently been used to map cerebral blood flow. We present an application of the technique using temporal contrast processing to image cerebral vascular structures with a field of view a few millimeters across an...

In fluorescent materials, energy from a certain band of incident wavelengths is reflected or reradiated at larger wavelengths, i.e. with lower energy per photon. While fluorescent materials are common in everyday life, they have received little attention in computer graphics. Especially, no bidirectional reflectance measurements of fluorescent materials have been available so far. In this paper...

Label-free imaging of individual viruses and nanoparticles directly in complex solutions is important for virology research and biosensing applications. A successful visualization technique should be rapid, sensitive, and inexpensive, while needing minimal sample preparation or user expertise. Current approaches typically require fluorescent labeling or the use of an electron microscope, which ...