A new scheme on test and control system for chlorophyll fluorescence is presented in this work, which uses light-emitting diode (LED) excitation by means of measuring the fluorescence parameter φpsII. The system takes programmable power supply as LEDs illumination drive power with high sensitivity and signal-tonoise ratio. MINIPAM is used to measure fluorescence parameter φpsII and keeps commun...

Understanding the complexity of the cellular environment will benefit from the ability to unambiguously resolve multiple cellular components, simultaneously and with nanometer-scale spatial resolution. Multicolor super-resolution fluorescence microscopy techniques have been developed to achieve this goal, yet challenges remain in terms of the number of targets that can be simultaneously imaged ...

Fluorescence spectroscopy has emerged in recent years as an effective way to detect cervical cancer. Investigation of the data preprocessing stage uncovered a need for a robust smoothing to extract the signal from the noise. Various robust smoothing methods for estimating fluorescence emission spectra are compared and data driven methods for the selection of smoothing parameter are suggested. T...

A photon-tissue interaction (PTI) model was developed and employed to analyze 96 pairs of reflectance and fluorescence spectra from freshly excised human pancreatic tissues. For each pair of spectra, the PTI model extracted a cellular nuclear size parameter from the measured reflectance, and the relative contributions of extracellular and intracellular fluorophores to the intrinsic fluorescence...

We report a novel method for estimating fluorescence impulse response function (fIRF) from noise-corrupted time-domain fluorescence measurements of biological tissue. This method is based on the use of high-order Laguerre basis functions and a constrained least-squares approach that addresses the problem of overfitting due to increased model complexity. The new method was extensively evaluated ...

Interaction of bovine erythrocyte spectrin with aminophospholipid (phosphatidylethanolamine, phosphatidylserine and their mixture) vesicles was studied by means of intrinsic fluorescence quenching and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene. Similarly as human and pig erythrocyte spectrin, bovine red blood cell spectrin interacts with vesicles prepared from these phospholipid...