Taurine was measured in cerebrospinal fluid by reacting it with fluorescamine to form a fluorescent derivative, followed by separation on a reversed-phase column and fluorometric detection and evaluation. The assay is rapid (17 min) and sensitive to as little as 1 mumol/L. The mean value for 27 cerebrospinal fluid samples collected from patients free from meningitis and aneurysm was 5.7 +/- 1.8...

Because of the primary aminic character of asulam, it is readily labelled with fluorescamine. A study of the variables affecting the derivatization reaction has been made. Two analytical approaches, that is, direct, synchronous spectrofluorimetry have been tested, and they both show good analytical performances. The linear dynamic range of the synchronous approach is 0.043-0.214 mug/ml and the ...

A fllwrometric amino acid analyzer using fllmresramine for the assay of the full array of natural amino ac<ids including prolinc on a single column is reported. The proline determination was carried out by specific introduction of a solution of ,\‘chlorosl~ccinimide into the flow system. Single rolumn fluorometric amino acid analysis was curried out in a significantly shorter time and with a se...

Corn (Zea mays L.) root tonoplast vesicles were treated with the primary-amine specific reagent, fluorescamine (FL). Modification by FL caused a differential inhibition to the coupled activities of tonoplast H(+)-ATPase. Within the range of 0 to 5 micromoles of FL per milligram of protein, the proton pumping rate was significantly reduced but ATP hydrolysis was only slightly affected. Yet, the ...

A simple spectrofluorimetric analysis of a local anaesthetic named procaine using a specific labelling reagent for primary amino groups, has been developed. Because procaine shows very weak native fluorescence, the technique of spectrofluorimetry has been very much limited for its determination. A detail study of the variables affecting the derivatisation reaction (pH, fluorescamine (FC) concen...

Two kinds of aequorin-type photoproteins, i.e., halistaurin and phialidin, and four kinds of modified forms of aequorin, i.e., products of acetylation, ethoxycarbonylation, fluorescamine-modification and fluorescein labelling, were prepared. The modified forms of aequorin were more sensitive to Ca2+ than was aequorin in their Ca2+-triggered luminescence reactions, whereas halistaurin and phiali...

1. Stabler, T. V., and Siegel, A. L., Rapid liquid-chromatographic/fluorometric method for taurine in biological fluids, involving prederivatization with fluorescamine. Gun. Chem. 27, 1771 (1981). Letter. 2. Larsen, B., Grasso, D., and Chung, S., A rapid method of taurine quantitation using high performance liquid chromatography. J. Chromatogr. Sci. 18, 233-236 (1980). 3. Connolly, B. M., and G...