نتایج جستجو برای: flp cs

تعداد نتایج: 33218  

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2007
Tiewen Liu Kyuhyung Kim Chris Li Maureen M Barr

Caenorhabditis elegans male mating provides a powerful model to study the relationship between the nervous system, genes, and innate sexual behaviors. Male mating is the most complex behavior exhibited by the nematode C. elegans and involves the steps of response, backing, turning, vulva location, spicule insertion, and sperm transfer. Because neuropeptides are important neural regulators of ma...

Journal: :Bioorganic & medicinal chemistry letters 2009
Matthew D Shoulders Kimberli J Kamer Ronald T Raines

According to a prevailing theory, (2S,4R)-4-hydroxyproline (Hyp) residues stabilize the collagen triple helix via a stereoelectronic effect that preorganizes appropriate backbone torsion angles for triple-helix formation. This theory is consistent with the marked stability that results from replacing the hydroxyl group with the more electron-withdrawing fluoro group, as in (2S,4R)-4-fluoroproli...

Journal: :BioTechniques 2000
D E Sabath M H Shim

Flp recombinase has been used extensively for in vivo manipulation of eukaryotic DNA at specific sequences designated as FRT sites. We developed a method to use Flp-mediated recombination without the need for drug resistance or metabolic selection of cells in which recombination has occurred. We generated expression plasmids directing expression of fusion proteins consisting of Flp recombinase ...

Journal: :PLoS Genetics 2008
M. Wayne Davis J. Jason Morton Dana Carroll Erik M. Jorgensen

The FLP enzyme catalyzes recombination between specific target sequences in DNA. Here we use FLP to temporally and spatially control gene expression in the nematode C. elegans. Transcription is blocked by the presence of an "off cassette" between the promoter and the coding region of the desired product. The "off cassette" is composed of a transcriptional terminator flanked by FLP recognition t...

Journal: :Nucleic acids research 1996
F Buchholz P O Angrand A F Stewart

We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in ...

Journal: :Nucleic acids research 1992
G B Panigrahi L G Beatty P D Sadowski

The FLP protein of the 2 microns plasmid of Saccharomyces cerevisiae promotes conservative site-specific recombination between DNA sequences that contain the FLP recognition target (FRT). FLP binds to each of the three 13 base pair symmetry elements in the FRT site in a site-specific manner. We have probed both major and minor groove contacts of FLP using dimethyl sulphate, monoacetyl-4-hydroxy...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1988
M Jayaram K L Crain R L Parsons R M Harshey

The FLP "recombinase" of the 2-micron circle yeast plasmid can resolve synthetic FLP site-Holliday junctions. Mutants of the FLP protein that are blocked in recombination but are normal in substrate cleavage can also mediate resolution. The products of resolution by these mutants are almost exclusively nicked molecules with a protein-bound 3' end. There is no significant asymmetry in strand cle...

B. Farhadinia

Recently, Gasimov and Yenilmez proposed an approach for solving two kinds of fuzzy linear programming (FLP) problems. Through the approach, each FLP problem is first defuzzified into an equivalent crisp problem which is non-linear and even non-convex. Then, the crisp problem is solved by the use of the modified subgradient method. In this paper we will have another look at the earlier defuzzifi...

Journal: :Genes & development 1997
J Lee M Jayaram

Recombination catalyzed by the Flp site-specific recombinase involves breakage and joining of four DNA strands between two target substrates. The reaction is carried out in two steps of pairwise strand exchanges by a DNA-protein assembly in which four Flp monomers act cooperatively to execute strand cleavage and joining. Two models for recombination have been proposed. In the trimer model, the ...

Journal: :Protein science : a publication of the Protein Society 2006
Jia-Cherng Horng Ronald T Raines

The polyproline type II (PPII) helix is a prevalent conformation in both folded and unfolded proteins, and is known to play important roles in a wide variety of biological processes. Polyproline itself can also form a type I (PPI) helix, which has a disparate conformation. Here, we use derivatives of polyproline, (Pro)10, (Hyp)10, (Flp)10, and (flp)10, where Hyp is (2S,4R)-4-hydroxyproline, Flp...

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