نتایج جستجو برای: flaa typing
تعداد نتایج: 22465 فیلتر نتایج به سال:
Molecular strain typing is essential for deciphering the epidemiology of Campylobacter jejuni infections. We applied two different methods, multilocus sequence typing (MLST) and analysis of the flaA short variable repeat (SVR), to 289 isolates (163 human, 56 chicken, 34 raw milk, and 36 environmental water isolates) collected in the province of Québec, Canada, over 3 years; in addition, the ana...
An outbreak of Legionnaires' disease at a long-term care facility in Ontario, Canada from September to October 2005 resulted in the death of 23 residents and the illness of 112 other people. In response, molecular methods were developed to detect Legionella pneumophila in clinical lung samples and to subtype isolates from clinical and environmental samples. The targeted genetic loci included Le...
Three sets of primers were designed for PCR detection and differentiation of Campylobacter jejuni and Campylobacter coli. The first PCR assay was designed to coidentify C. jejuni and C. coli based on their 16S rRNA gene sequences. The second PCR assay, based on the hippuricase gene sequence, identified all tested reference strains of C. jejuni and also strains of that species which lack detecta...
The objective of this study was to investigate the genotypic characteristics of Campylobacter isolates in Shiraz, Iran. A total of 40 Campylobacter isolates including 20 C. jejuni and 20 C. coli were recovered from both cattle and sheep faeces by cultivation methods. The isolates were identified on the basis of polymerase chain reaction (PCR) detection of 16SrRNA and multiplex PCR to determine ...
The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jejuni and Campylobacter coli. High-resolution melting (HRM) analysis is emerging as an efficient and robust method for discriminating DNA sequence variants. The objective of this study was to apply HRM analysis to flaA-based genotyping. The initial aim was to identify a suitable flaA fragment. It w...
We present an optimized multilocus sequence typing (MLST) scheme with universal primer sets for amplifying and sequencing the seven target genes of Campylobacter jejuni and Campylobacter coli. Typing was expanded by sequence determination of the genes flaA and flaB using optimized primer sets. This approach is compatible with the MLST and flaA schemes used in the PubMLST database and results in...
زمینه: هلیکوباکتر پیلوری شایع‌ترین باکتری است که جوامع انسانی را در ابعاد جهانی مبتلا به عفونت ساخته است. بیان فلاژل و تحرک باکتری در کلونیزاسیون و ویرولانس آن عامل بسیار مهمی است. ژن flaA یکی از ژن‌های کد کننده فلاژلین می‌باشد که نقش کلیدی در حرکت باکتری و کلونیزاسیون آن دارد و از نظر ایمنی بخشی نیز مورد توجه می‌باشد. هدف از این مطالعه طراحی، ساخت سازواره و بررسی بیان ژن flaA هلیکوباکتر پیلوری...
In the presented paper, PCR-RFLP and RFLP-PFGE with two different enzymes were applied for the discrimination of 48 Campylobacter isolates from poultry carcasses and faeces. Both used typing methods had a typeability of 100% on this collection of the strains. PFGE with SacII enzyme was the most discriminatory method with the discrimination index of 0.98. Restriction analysis of the flaA gene al...
FlaA was recently found to be associated with flagellar filaments of Borrelia burgdorferi. We tested whether antibodies to this protein are a good indicator of infection, as antibodies to FlaA proteins in other spirochetal infections show an increase in titer. Although overproduction of intact FlaA was highly toxic to Escherichia coli, truncated proteins which lacked the N-terminal signal seque...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید