Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evalu...

Semen for insemination, either intrauterine or in vitro, must be prepared to remove seminal plasma products and/or select the healthier population of sperm prior to use. Traditionally, a double wash technique is performed, with or without subsequent swim-up to isolate the motile fraction if necessary. More recently, the use of the SperPrep filtration method has gained acceptance, with the benef...

although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. the methods of sperm separation that have been repeatedly reported to isolate fractions rich in x- or y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. however, the ability of the se...

X and Y chromosome paints were developed from sorted yak chromosomes for sexing cattle spermatozoa. Clear hybridization signals were obtained for every spermatozoon using a modified sperm decondensation protocol and fluorescence in situ hybridization (FISH). The procedure was evaluated using the established Beltsville sperm sexing technology, which separates spermatozoa by flow cytometry into X...

Objective The aim of this study was to evaluate the non-genomic action of thyroxin on sperm kinetic and its probable use to improve sperm recovery after applying an enrichment method like “swim-up” in comparison with the available one, pentoxifylline. MaterialsAndMethods This is an experimental study. A total of 50 patients were recruited, followed by infertility consultation. Conventional sper...

objective: selection of sperm for intra-cytoplasmic sperm injection (icsi) is usually considered as the ultimate technique to alleviate male-factor infertility. in routine icsi, selection is based on morphology and viability which does not necessarily preclude the chance injection of dna-damaged or apoptotic sperm into the oocyte. sperm with high negative surface electrical charge, named “zeta ...

Preconceptual sex selection is still a highly debatable process whereby X- and Y-chromosome-bearing spermatozoa are isolated prior to fertilization of the oocyte. Although various separation techniques are available, none can guarantee 100% accuracy. The aim of this study was to separate X- and Y-chromosome-bearing spermatozoa using methods based on the viability difference between the X- and Y...

OBJECTIVE This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals. METHODS Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sper...

BACKGROUND The aim of this study was to evaluate the non-genomic action of thyroxin on sperm kinetic and its probable use to improve sperm recovery after applying an en- richment method like "swim-up" in comparison with the available one, pentoxifylline. MATERIALS AND METHODS This is an experimental study. A total of 50 patients were re- cruited, followed by infertility consultation. Conventi...