It is known that the follicular selection and final stages of follicular maturation are equal if not more dependent on low circulating levels of LH.In addition to stimulating production of thecal androgens as substrate for estrogen synthesis, LH stimulates granulose cell via LH receptors induced by FSH and estrogen in larger but not smaller follicles. The data from numerous assisted reproductio...

To evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did...

the study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gff) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in black bengal goats. follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. media were prepared using tcm-199 supplemented wi...

oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. progesterone (p4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

Human chorionic gonadotropin (hCG) has been used as a surrogate for the mid-cycle LH surge for several decades. Due to structural and biological similarities with LH, hCG binds to and activates the same receptor - the LH/hCG receptor. However, despite the fact that hCG effectively secures final oocyte maturation and ovulation, its use as a surrogate for LH has got several drawbacks - first and ...

BACKGROUND Ovarian follicle growth and maturation requires extensive communication between follicular somatic cells and oocytes. Recently, intercellular cell communication was described involving cell-secreted vesicles called exosomes (50-150 nm), which contain miRNAs and protein, and have been identified in ovarian follicular fluid. The goal of this study was to identify a possible role of exo...

Follicular fluid (FF) provides a very important microenvironment for the development of oocyte maturation, ovulation and fertilization. The objective of this study was to determine the effect of different charged groups of cow follicular fluid proteins on in vitro oocyte maturation (IVM) when added to cultured maturation medium. The follicular fluid were obtained from dominant follicle and prec...

The aim of this study was to evaluate the effects of ovarian tissue vitrification and two-step in vitro culture on the metaphase II (MII) oocyte reactive oxygen species (ROS) level, mitochondrial transcription factor A (TFAM) expression and succinate dehydrogenase (SDH) activity. After collection of neonatal mouse ovaries, 45 ovaries were vitrified and the others (n = 45) were...

Oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. Progesterone (P4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

Background The purpose of triggering in ovulation induction is to induce the final maturation of oocytes and their release from the ovary for fertilization. Objective The aim of the present study was to compare the effectiveness of gonadotropin-releasing hormone (GnRH) agonist and human chorionic gonadotropin (HCG) on the final maturation of oocytes and pregnancy rates in intrauterine insemin...