Bone marrow mononuclear cells (BMNCs) are widely used in regenerative medicine, but recent data suggests that the isolation of BMNCs by commonly used Ficoll-Paque density gradient centrifugation (DGC) causes significant cell loss and influences graft function. The objective of this study was to determine in an animal study whether and how Ficoll-Paque DGC affects the yield and composition of BM...

The separation of lymphocytes from the other blood cells is based on the differences in their specific gravity. Centrifugation of whole blood against an isopaque-ficoll gradient results in the formation of three separate layers: the top one contains the slowly sedimenting cells (lymphocytes, platelets and some monocytes); the inter­ mediate one, the granulocytes; the bottom layer contains the e...

The cellular requirements for the primary in vitro IgM and IgG response to dinitrophenyl-substituted Ficoll were examined. Neither thymus-derived lymphocytes nor macrophage-rich splenic adherent cells were required for anti-DNP antibody synthesis. DNP-Ficoll is therefore tentatively classified as a "thymic-independent" antigen.

The present study has examined the possibility of TNP-Ficoll-responsive B cells recognize the MHC determinants expressed by the accessory cells with which they interact for the generation of T cell-independent responses to "high" concentrations (10(-2) micrograms/ml) of TNP-Ficoll. In experiments with B cells from normal mice, it was found that MHC homology between the TNP-Ficoll-responsive B c...

Levitt et aP and Greenberg et al’ have recently described elegant immunologic methodologies for selectively isolating T lymphocytes’ or hematopoietic progenitor cells2 from small aliquots of human marrow. In both cases the initial marrow manipulation, following aspiration, involved selective isolation of light-density mononuclear cells using Ficoll-Hypaque (I .077 ± 0.003 g/dL) gradients. Poten...