Hydroxylation of 5 beta-[7 beta-3H]cholestane-3 alpha, 7 alpha-diol was studied in mitochondrial preparations from human fetal livers. The livers were obtained at legal abortions between weeks 14 and 24. In addition to hydroxylation in the 26-position, 5 beta-cholestane-3 alpha, 7 alpha-diol was hydroxylated in the 12 alpha-position. In one experiment, mitochondrial protein was solubilized and ...

in this study the uptake and metabolism of adenosine by mitochondria has been investigated. incubation of cem cells mitochondria preparation with [3h] -adenosine showed substantial uptake and metabolism of adenosine. adenosine was both anabolized to amp, adp and atp, and also catabolized to inosine. the highest concentration of metabolites in extracted mitochondria was due to amp. the mitochond...

Intra-uterine growth retardation has been linked to the development of type 2 diabetes in later life. Mitochondrial changes have been suggested as a link between fetal malnutrition and adult insulin resistance. Taurine has been implicated in this process. We investigated whether protein malnutrition in early life alters mitochondria of the pancreatic islets in adulthood, and whether taurine sup...

In adult regenerating cardiomyocytes in culture, in contrast to fetal cells, mitochondrial creatine kinase (Mi-CK) was expressed. In the same cell, two populations of mitochondria, differing in shape, in distribution within the cell and in content of Mi-CK, could be distinguished. Immunofluorescence studies using antibodies against Mi-CK revealed a characteristic staining pattern for the two ty...

In this study the uptake and metabolism of adenosine by mitochondria has been investigated. Incubation of CEM cells mitochondria preparation with [3H] -adenosine showed substantial uptake and metabolism of adenosine. Adenosine was both anabolized to AMP, ADP and ATP, and also catabolized to inosine. The highest concentration of metabolites in extracted mitochondria was due to AMP. The mito...

The relationship between arterial and tissue PO2 is not well understood in the adult, let alone in the fetus. Figure 1 shows the pathway for oxygen through the maternal lungs and pulmonary circulation to the systemic circulation. For the fetus, there is an additional step of transfer across the placenta to fetal blood, and thence via the fetal heart to systemic circulation and fetal tissues. It...

mitochondria are unique organelles with major role in cellular function, not only as a major site of atp production, but also by regulating energy expenditure, apoptosis signaling, and production of reactive oxygen species. they play a central role in determining the point-of-no-return for the apoptotic process. there is accumulating evidence supporting a direct link between mitochondria, oxida...

pioglitazone (pg) is one of thiazolidinediones used for the treatment of type ii diabetes mellitus. some reports of its hepatotoxicity exist, but the mechanism of its hepatotoxicity is not well known. in the present study, the protective effect of some atp suppliers are investigated against mitochondrial toxicity of pg in isolated rat mitochondria. mitochondrial viability was investigated by mt...

background: friedreich ataxia (frda) is an autosomal recessive disorder caused by guanine-adenine-adenine (gaa) triplet expansions in the fxn gene. its product, frataxin, which severely reduces in frda patients, leads to oxidative damage in mitochondria. the purpose of this study was to evaluate the triple nucleotide repeated expansions in iranian frda patients and to elucidate distinguishable ...

background: the mitochondria are an important source of adenosine triphosphate (atp) production in pre-implantation embryo. therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and atp content. methods: the embryos at 2-pn, 4-cell and blastocyst stages were collected from the oviduct of st...