By using representative strains of Chlamydia trachomatis serotypes, the efficacy of substituting fetal bovine serum with newborn calf serum for the isolation of this organism in tissue culture was examined. The criteria used were the quality and quantity of the iodine-stained cytoplasmic inclusions and the characteristics of the McCoy cells. Complete substitution of fetal bovine serum with newb...

The effects of the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on the morphology and growth properties of C3H/10T1/2 clone 8 cells were examined. The morphology of these cells was changed within 30 min following treatment with 0.1 microgram of TPA per ml; they became smaller and refractile with long beady processes. Such changes were observed in both logarithmic and...

OBJECTIVE To determine if insulinlike growth factor I (IGF-I) and basic fibroblast growth factor (bFGF), individually or in combination, support the growth and viability of human septal chondrocytes in a serum-free medium (SFM) and a serum-enhanced culture medium. DESIGN Chondrocytes were recovered from enzymatically digested human septal cartilage and were plated for monolayer culture in a n...

BACKGROUND Batch variability of sera used for cell culture is of considerable experimental concern. A novel fetal calf serum product, FCS Gold, was claimed to be the first defined fetal calf serum free of batch variation. MATERIALS AND METHODS The efficacy of methotrexate (MTX) and LY231514 (multitargeted antifolate, MTA) in CCRF-CEM cells and KB cells was compared using media supplemented wi...

Human fibroblast strains, 48BR, GMO73OA, and IBR/3 (from the MRC Cell Mutation Unit, Brighton, England), were obtained from skin biopsy of normal adult individuals aged 46 (female), 45 (female), and 33 (male), respectively. EK13 and EK4 fibroblasts were derived from foreskin explants in this laboratory(see below). Fibroblast cultures were grown in Earle's modified MEM (Gibco) supplemented with ...

The growth responses of 5-deoxy-5-methylthioribose on a 5'-deoxy-5'-methylthioadenosine phosphorylase containing cell line (BW5147) and the methylthioadenosine phosphorylase-deficient cell line (L1210D) were examined. Methylthioribose was shown to dramatically affect these cells, increasing their growth rate, saturation density, and viability. It was also found that methylthioribose could satis...

Genus Leishmania is a protozoan parasite that causes different severe diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, for mass cultivation and the most expensive ingredient in these media. In the present work, the efficacy of chicken serum was evaluated in Leishmania culture media. The results indicated that, the (10%) chicken serum enriched culture medium su...

Many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. The proliferation and expansion of human hematopoietic stem cells in ex vivo culture were examined with the goal of generating a suitable protocol for expanding hematopoietic stem cells for patient transplantation. Using primary fetal liver cells, we established...