A marker's dark triplet state is of great importance in fluorescence microscopy: It serves as a means to switch off fluorescent markers and is thus the enabling element for several super-resolution methods. On the other hand, intersystem-crossing to the electronic dark triplet state strongly reduces the fluorescence yield in conventional fluorescence microscopy. The ability to determine the kin...

Mass spectrometric (MS) data of human cell secretomes are usually run through the conventional human database for identification. However, the search may result in false identifications due to contamination of the secretome with fetal bovine serum (FBS) proteins. To overcome this challenge, here we provide a composite protein database including human as well as 199 FBS protein sequences for MS ...

Background: Follicle stimulating hormone (FSH) is one of the most common and effective gonadotropin hormones used in infertility treatment. Production of FSH as a drug is affordable as long as it is done with minimal cost. FBS is the most expensive component in medium culture furthermore; it’s being controversial to cause unnecessary suffering for the unborn calf for collection their serum. In ...

To each flat conformal structure (FCS) of hyperbolic type in the sense of Kulkarni-Pinkall, we associate, for all θ ∈ [(n − 1)π/2, nπ/2[ and for all r > tan(θ/n) a unique immersed hypersurface Σr,θ = (M, ir,θ) in H of constant θ-special Lagrangian curvature equal to r. We show that these hypersurfaces smoothly approximate the boundary of the canonical hyperbolic end associated to the FCS by Kul...

BACKGROUND Apoptosis is thought to be a critical component of disc degeneration. Two main pathways of Fas-mediated apoptosis have been identified: Type I, which is the death-inducing signaling complex pathway, and Type II, which is the mitochondrial pathway. The apoptotic pathway for anulus fibrosus cells, which is phenotypically different from that of nucleus pulposus cells, has not been eluci...

Insulin-like growth factors I and I1 (IGF-I and IGF11) were prepared from fetal calf serum and adult bovine serum by gel filtration, immunoaffinity chromatography, chromatofocusing, and reverse-phase high pressure liquid chromatography, and their complete amino acid sequences determined. IGF-I and -11 are found in both adult and fetal serum. The sequence of bovine IGF-I is found to be identical...

The yields of the Armstrong and WE strains of lymphocytic choriomeningitis virus in baby hamster kidney (BHK) cells cultivated in either bovine, calf, fetal bovine, or horse serum were investigated. Lines of BHK cells were established in these sera. When the infected cell lines were observed by immunofluorescence, the per cent fluorescing cells for a given virus strain did not vary. However, fo...

Fetal bovine serum (FBS) and adult bovine serum (BS) exhibited bactericidal activity against Helicobacter pylori at various levels, which were higher in BS than in FBS. The bactericidal activity was inactivated by heat treatment at 56 degrees C for 30 min. Our results demonstrated that heat-treated BS is a useful serum source of H. pylori culture medium.