The use of serologically detectable male (SDM; also called H-Y) antigens to identify male embryos may be limited by the source of anti-SDM antibody. In the present study, novel anti-SDM B9-Fab recombinant clones (obtained by chain shuffling of an A8 original clone) were used to detect SDM antigens on murine embryos. Murine morulae and blastocysts (n=138) were flushed from the oviducts of Kunmin...

Studies of CO ligand binding revealed that two protein states with different ligand affinities exist in the protoglobin from Methanosarcina acetivorans (in MaPgb*, residue Cys(E20)101 was mutated to Ser). The switch between the two states occurs upon the ligation of MaPgb*. In this work, site-directed mutagenesis was used to explore the role of selected amino acids in ligand sensing and stabili...

Lymphocytes from b(5)/b(9) rabbits were stained in suspension with fluorescent antiallotype antibody reagents to selectively label with fluorescent molecules those cells bearing membrane immunoglobulin (Ig) of the b5 or b9 allotype. After staining, the cells were separated by the fluorescence-activated cell sorter into populations markedly enriched in cells bearing b5 or b9 membrane Ig or total...

Previous studies in rat bile canalicular membrane vesicles and WIF-B9 cells revealed that cAMP-induced trafficking of ATP-binding cassette (ABC) transporters to the canalicular membrane and their activation require phosphoinositide 3-kinase (PI3-K) products. In the present studies, canalicular secretion of fluorescein isothiocyanate-glycocholate in WIF-B9 cells was increased by cAMP and a decap...

In one carbon reaction, tetrahydrofolate works as cofactor for the synthesis of glycine, serine, methionine, purines and thymidylate. Folate or vitamin B9 is not synthesized in humans therefore plants are the main source of this essential vitamin. Deficiency of vitamin B9 may cause severe health consequences like birth defects, megaloblastic anemia, cardiovascular disorders, cancers, etc. To re...

The amino acid sequence of the constant (CK) region from the kappa immunoglobulin chains of a b9 rabbit is compared with the CK sequences, taken from the literature, of a b4 rabbit. These CK regions differ by 33% of their amino acid sequences and by three sequence insertions or deletions (sequence gaps). These extensive differences together with other published observations suggest that the b9 ...

Tillage practices and nitrogen (N) sources are important factors affecting rice production. Few studies, however, have examined the interactions between tillage practices and N fertilizer sources on NH3 volatilization, nitrogen use efficiency (NUE), and rice grain yield. This study aimed to investigate the effects of N fertilizer sources (no N fertilizer, inorganic N fertilizer, organic N ferti...

• Session 1: Objectives, outcomes, and trade-offs associated with alternative fertilizer promotion programmes. • Session 2: What determines the cost of fertilizer at the farm gate? Cost build-up analysis (Bumb, g p y (IFDC) • Session 3: Fertilizer profitability analysis (Rashid and Jayne, IFPRI/MSU) • Session 4: Experiences and lessons learned with alternative fertilizer promotion efforts (Mino...

Amaç: B grubu vitaminler ve D vitamini eksikliği/yetersiz alımının depresyonla ilişkili olduğu gösterilmiştir. Önceki çalışmalar sadece folat (B9) B12 vitaminleri veya vitaminine odaklanmıştı, bu çalışmada ise B1 B2 B9 vitaminlerinin serum diyetle alım düzeyleri araştırılmıştır. Bu nedenle çalışmanın amacı, bazı vitaminlerin vitamininin üniversite öğrencilerinin depresyon düzeylerine etkisini a...