Procedures for fermentative determination, in blood and urine, of glucose, or of sugars resembling it in their fermentability by yeast, have been refined by different investigators during the past few years. The chief source of error, undue prolongation of the time of fermentation to one or more days, was removed by Hiller, Linder, and Van Slyke (1925), who utilized conditions under which gluco...

BACKGROUND Intermediates from processing sugar beets are considered an attractive feedstock for ethanol fermentation due to their high fermentable sugar content. In particular, medium prepared from raw sugar beet juice seems to be suitable for use in fermentation processes, but it is microbiologically unstable and requires sterilization. RESULTS This study investigates the effect of ozone tre...

Using 16S rRNA gene-based approaches, we analyzed the responses of ileal and colonic bacterial communities of weaning piglets to dietary addition of four fermentable carbohydrates (inulin, lactulose, wheat starch, and sugar beet pulp). An enriched diet and a control diet lacking these fermentable carbohydrates were fed to piglets for 4 days (n = 48), and 10 days (n = 48), and the lumen-associat...

Fermentable carbohydrates (FC) are a major cost component and environmental burden in the production of biofuels bio-based commodity chemicals; consequently, FC determines economic feasibility performance chemicals. Producing with conventional technology without additional processing steps food-grade sugar can result low-cost industrial-grade fermentable that is competitive raw sugar. This stud...

BACKGROUND The use of Ionic liquids (ILs) as biomass solvents is considered to be an attractive alternative for the pretreatment of lignocellulosic biomass. Acid catalysts have been used previously to hydrolyze polysaccharides into fermentable sugars during IL pretreatment. This could potentially provide a means of liberating fermentable sugars from biomass without the use of costly enzymes. Ho...

BACKGROUND The inevitable depletion of fossil fuels has resulted in an increasing worldwide interest in exploring alternative and sustainable energy sources. Lignocellulose, which is the most abundant biomass on earth, is widely regarded as a promising raw material to produce fuel ethanol. Pretreatment is an essential step to disrupt the recalcitrance of lignocellulosic matrix for enzymatic sac...

The oleaginous yeast Yarrowia lipolytica is an industrially important host for production of organic acids, oleochemicals, lipids, and proteins with broad biotechnological applications. Albeit known for decades, the unique native metabolism of Y. lipolytica for using complex fermentable sugars, which are abundant in lignocellulosic biomass, is poorly understood. In this study, we activated and ...