The femAB operon codes for two nearly identical approximately 50-kDa proteins involved in the formation of the staphylococcal pentaglycine interpeptide bridge. Sequencing and analysis of the femA region of mutants isolated by chemical mutagenesis and selection for lysostaphin resistance revealed point mutations leading to the expression of truncated FemA proteins. These femA mutants, although s...

Fem proteins are the essential structural proteins of various gram-positive bacteria. These are of three different types namely FemX (FmhB), FemA and FemB. Only two Fem protein crystallographic structures are available till date, one for FemA in Staphylococcus aureus and another for FemX in Weissella viridescensis. In this study, computational methods are used to evaluate interaction of FemA pr...

Protein-protein interactions play an important role in all cellular processes. The development of two-hybrid systems in yeast and bacteria allows for in vivo assessment of such interactions. Using a recently developed bacterial two-hybrid system, the interactions of the Staphylococcus aureus proteins FemA, FemB and FmhB, members of the FemABX protein family, which is involved in peptidoglycan b...

The most commonly used analysis method in performance-based design (PBD) is nonlinear static (NSA). In unsymmetrical 2D frames, unlike for the symmetrical state, NSA should be performed two lateral loading directions. This complicates process of achieving a feasible optimal addition to increasing volume calculations. this study, overcoming issue, two-step approach proposed PBD steel moment-resi...

Nepal is situated in an area with considerable seismic activity. It important to choose structural systems that can withstand the lateral loads. In a dual system of reinforced concrete structures, placement shear walls optimally increases stiffness buildings. The rigidity RC buildings may be improved by strategically placing lift core walls. Buildings would more useful if they had easy access e...