Dynamic changes of physiological bioprocess parameters, e.g. a change in the specific growth rate μ, are frequently observed during industrial manufacturing as well as bioprocess development. A quantitative description of these variations is of great interest, since it can bring elucidation to the physiological state of the culture. The goal of this contribution was to show limitations and issu...

The Distributed Batch Controller (DBC) supports scientiic batch data processing. The DBC distributes batch jobs to one or more pools of workstations and monitors and controls their execution. The pools themselves may be geographically distributed, and need not be dedicated to processing batch jobs. We describe the use of the DBC in a large scientiic data processing application, namely the gener...

BACKGROUND Enzymatic hydrolysis, the rate limiting step in the process development for biofuel, is always hampered by its low sugar concentration. High solid enzymatic saccharification could solve this problem but has several other drawbacks such as low rate of reaction. In the present study we have attempted to enhance the concentration of sugars in enzymatic hydrolysate of delignified Prosopi...

Nonoverlapping batch means (NOLBM) is a a we11-known approach For For estimating the variance of the sample mean. In this paper we consider an overlapping batch means (OLBM) estimator that, based on the same assumptions and batch size as as NOLBM, has has essentially the same mean and only 2/3 the asymptotic variance of NOLBM. Confidence interval procedures for the nlean based on NOLBM and OLBM...

Fed-batch culture strategy is often used for increasing production of heterologous recombinant proteins in Escherichia coli. This study was initiated to investigate the effects of dissolved oxygen concentration (DOC), complex nitrogen sources and pH control agents on cell growth and intracellular expression of streptokinase (SK) in recombinant E. coli BL21(DE3). Increase in DOC set point from 3...

In this paper optimal control algorithms for two E. coli fed-batch fermentations are developed. Fed-batch fermentation processes of E. coli strain MC4110 and E. coli strain BL21(DE3)pPhyt109 are considered. Simple material balance models are used to describe the E. coli fermentation processes. The optimal feed rate control of a primary metabolite process is studied and a biomass production is u...

Batch-to-batch iterative learning control of a fed-batch fermentation process using batchwise linearised models identified from process operation data is presented in this paper. Due to model-plant mismatches and the present of unknown disturbances, off-line calculated control policy may not be optimal when implemented to the real process. The repetitive nature of batch process allows informati...

Alkali-pretreated sugarcane bagasse fiber was subjected to fed-batch simultaneous saccharification and fermentation (SSF) with a prehydrolysis process to increase the solids loading and produce a high concentration of ethanol. The hydrolysis medium and yeast feeding modes were investigated to determine suitable conditions for high sugar yield and ethanol production. Batch addition resulted in a...

BACKGROUND To make lignocellulosic fuel ethanol economically competitive with fossil fuels, it is necessary to reduce the production cost. One way to achieve this is by increasing the substrate concentration in the production process, and thus reduce the energy demand in the final distillation of the fermentation broth. However, increased substrate concentration in simultaneous saccharification...

Cephalosporin C (CPC), a β-lactam antibiotic, is starting molecule for industrial production of semi synthetic cephalosporins. CPC fermentation has been carried out in Air Lift Reactor (indigenously made up of borosilicate glass) in different fermentation modes i.e., batch and fed batch modes using a mold Acremonium chrysogenum (synn. Cephalosporium acremonium). A definite correlation exists be...