background : in this study, we used both its1 and its2 for molecular identification of fasciola species. methods : the region between 18s and 28s of ribosomal dna was used in pcr-rflp method for molecular identification of fasciola species. ninety trematodes of fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from khorasan, east azerbaijan, a...

BACKGROUND The trematodes of the genus Fasciola (the liver flukes) are among the well-known instances of food-borne parasites worldwide. Differentiation of Fasciola species is important because of their different transmission and epidemiological characteristics. The current study was undertaken to discriminate Fasciola species in the domestic ruminants of Urmia city, Iran. METHODS Adult fluke...

Fasciola parasites (liver flukes) express numerous cathepsin L proteases that are believed to be involved in important functions related to host invasion and parasite survival. These proteases are evolutionarily divided into clades that are proposed to reflect their substrate specificity, most noticeably through the S(2) subsite. Single amino acid substitutions to residues lining this site, inc...

Development and evaluation of a single step duplex PCR for simultaneous 1 detection of Fasciola hepatica and Fasciola gigantica 2 (Fasciolidae; Trematoda; Platyhelminthes) 3 Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, 4 Xuyen Thi Kim Le, Chau Thi Minh Hoang, and Nguyen Van De 5 Department of Immunology, Institute of Biotechnology, Vietnam Academy of Science and 6 T...

Prevalence of Fasciola hepatica L. infection among cattle slaughtered in the Lublin province in the years 2005-2008 was evaluated. The study draws its data from annual reports of the Veterinary Inspectorate in Lublin. The results of the study are as follows: the prevalence of Fasciola hepatica infection in the cattle of the Lublin province between 2005 and 2008 amounts to 21.24%.

Prevalence of Fasciola hepatica L. infection among cattle slaughtered in the Lublin province in from 2009-2012 was evaluated. The data were taken from annual reports of the Veterinary Inspectorate in Lublin. The results of the study are as follows: the prevalence of Fasciola hepatica infection in the cattle of the Lublin province between 2009 and 2012 is found to be 11.97%.

BACKGROUND The purpose of this comparative study was to detect superoxide dismutase (SOD) activities in Fasciola hepatica, F. gigantica parasites, infected and healthy liver tissues in order to determine of species effects and liver infection on SODs activity level. METHODS Fasciola spp. parasites and sheep liver tissues (healthy and infected liver tissues), 10 samples for each, were collecte...

Fasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag) and Crude (Cr Ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. For this...

A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of ...

Two monoclonal antibodies to Fasciola gigantica excretory/secretory (ES) antigens were used in a sandwich ELISA for the detection of Fasciola antigens in faeces of 9 sheep experimentally infected with 300 metacercariae of F. gigantica. The detection of coproantigens was found in four of the seven sheep within 5 weeks of infection, and within 7 weeks of infection coproantigens were detected in a...