Identification of Mycobacterium tuberculosis and Rifampin Resistance in Pulmonary Extra-pulmonary Clinical Specimens Using the Gene Xpert MTB/RIF Assay

Tuberculosis is one of the most important infectious diseases. In 2009 estimate 9 million new cases were registered worldwide, mortality due tuberculosis reached 1.5 million (Markowski et al., 2011). Tuberculosis may infect any part of the body, but most commonly occurs in the lungs (known as pulmonary tuberculosis) (Dolin et al., 2010). Extra pulmonary TB occurs when tuberculosis develops outs...

BACKGROUND & OBJECTIVES As isolation of Mycobacterium tuberculosis in culture requires a long time, there is a need for simple rapid method for direct detection of M. tuberculosis from clinical specimens. Amplified nucleic acid hybridization assays such as polymerase chain reaction (PCR) have shown promising results. In the present study, the sensitivity of PCR assay was assessed over smear mic...

Tuberculosis is a chronic, granulomatous disease. Primary lesion usually occurs in the lung. Extra pulmonary infection commonly involves head, neck and abdomen. In the absence of typical features of tuberculosis, tuberculous cellulitis mimicking oral infection may pose a challenge for diagnosis. In an attempt to highlight an uncommon presentation, we document a case of extra pulmonary tuberculo...

Background: Tuberculosis has been detected in some extra pulmonary ecological niches. Although extra pulmonary tuberculosis (EPTB) is less frequent than Pulmonary Tuberculosis (PTB), its incidence has increased worldwide. The aim of this study was to investigate the presence of EPTB and MDR-EXPT in Formalin-fixed, paraffin-embedded tissue blocks among different samples in Kerma...

Introduction Tuberculosis, one of the oldest diseases is known to affect human and is caused by mycobacterium tuberculosis. The disease may be fatal within the 5 years in more than 50% of cases (1). Genitourinary tuberculosis accounts for about 15% of all extra pulmonary tuberculosis; this is usually due to hematogenous spread following primary infection and may involve any portion of genitouri...

Background: Although infectious diseases are the most common sources for the fever of unknown origin (FUO), but the spectrum of infectious diseases is changing overtime. The purpose of the study was to define the clinical spectrum and changing the pattern of FUO.Methods: This existing data based study was undertaken from 2007 to 2011. One hundred-six patients fulfilling the modified criteria fo...

Mycobacterium tuberculosis, the causative agent of tuberculosis was discovered by Robert Koch in 1882. It has been observed that 30% of the world population harbours Mycobacterium tuberculosis. Spread of Human immunodeficiency virus (HIV) infection, malignancies, globalization and malnutrition are some of the major contributing factors for increase in the global burden of tuberculosis (pulmonar...

Tuberculosis is a major health problem throughout the world. Inhalation is the most susceptible pathway of Mycobacterium tuberculosis infection, pulmonary tuberculosis being the predominant one. Apart from this, Extra Pulmonary Tuberculosis may also arise as a result of the reactivation of tuberculous focus after dissemination from a chief focus, being a manifestation of tuberculosis infecting ...