Exogenous protein expression in mammalian cells is necessary to produce therapeutic proteins and modern medical applications like developing DNA vaccines gene therapy. This study examines the human-Globin (hBG) second intron's capacity for intron-mediated enhancement (IME) various cell lines. Our study's main aim investigate effect of incorporation arrangement intron human Beta-globin into pVAX...

Neurogenin 2 encodes a neural-specific transcription factor (NGN2) able to drive neuronal fate on somatic and stem cells. NGN2 is expressed in neural progenitors within the developing central peripheral nervous systems. Overexpression of human induced pluripotent cells (hiPSCs) or embryonic has been shown efficiently trigger conversion neurons. Here we describe two gene-edited hiPSC lines harbo...

The applicability of tightly regulated transgenesis in domesticated animals is severely hampered by the present lack of knowledge of regulatory mechanisms and the long generation intervals. To capitalize on the tightly controlled expression of mammalian genes made possible by using prokaryotic control elements, we have used a single-step transduction to introduce an autoregulative tetracycline-...

results a significant increase in blood abca1 (ex group p < 0.002, t = - 9.876) and apo a-i (ex group p < 0.05, t = 2.76) gene expression was shown following the 12 weeks of training. plasma high-density lipoprotein-cholesterol (hdl-c) concentration increased (p < 0.001, t = 4.90 respectively) while plasma low-density lipoprotein-cholesterol (ldl-c) concentration decreased (p < 0.001, t = 4.27)...

While naked DNA gene transfer in vivo usually results in transient gene expression, in some cases long-term transgene expression can be achieved. Here we demonstrate that cis-acting DNA elements flanking the transgene expression cassette and components in the plasmid backbone can significantly influence expression levels from nonviral vectors. To demonstrate this, we administered our most robus...

We report here an alternative and easy approach to generate sufficient quantity of minimal gene expression cassette (promoter–open reading frame–terminator) DNA by PCR amplification using high fidelity DNA polymerases. Isolating the minimal gene cassette DNA in large quantities by restriction digestion and gel extraction for biolistic-mediated ‘clean DNA’ transformation, is not only a cumbersom...

BACKGROUND The most widespread, efficient prokaryotic protein-producing system is one where the T7 phage polymerase recognizes the T7 phage promoter (T7 p/p system). Unfortunately, in this system, target protein expression gradually declines and is often undetectable following 3 to 5 subcultures. Although a number of studies have attempted to stabilize the expression levels of the T7 p/p system...

A gene cassette incorporating the crs-rsd site-specific recombination system from the Salmonella enterica subsp. enterica serovar Dublin virulence plasmid improved the inheritance in S. enterica serotype Typhi strain CVD908-htrA of a multicopy plasmid expression vector. Use of this recombination cassette may improve expression of heterologous antigens from multicopy plasmid expression vectors i...

tissue plasminogen activator (tpa) is a serine protease, which is composed of five distinct structural domains with 17 disulfide bonds, representing a model of high-disulfide proteins in human body. one of the most important limitations for high yield heterologous protein production in escherichia coli (e. coli) is the expression of complex proteins with multiple disulfide bridges. in this stud...

BACKGROUND The use of small interfering RNAs (siRNAs) as genetic inhibitors of gene expression has been shown to be an effective way of studying gene function in mammalian cells. Recently, different DNA vectors for expression of small hairpin RNAs (shRNAs) or co-expression of sense and antisense RNAs have been developed that direct siRNA-mediated gene silencing. One expression cassette design t...