Background: DNA immunization is an appropriate method to produce an immunological response. Pseudomonas aeruginosa produces exotoxin A which is highly cytotoxic for eukaryotic cells. Since domains II (translocation domain) and 1b of the toxin have antigenic qualities, so they could be  useful candidates to protect against pseudomonas infections. Objectives: To evaluate if recombinant plasmid co...

pseudomonas aeruginosa as an opportunistic pathogen produces several virulence factors. the most important of these factors are exotoxin a and type iii secretion system (t3ss). the aim of this study was to determine the frequency of toxa, exou and exos genes among clinical isolates of p. aeruginosa. in this cross-sectional study from september 2011 to february 2012, 156 p. aeruginosa isolates w...

The determinants necessary for adaptation to high NaCl concentrations and competition for nodule occupancy in Sinorhizobium meliloti were investigated genetically. Mutations in fabG as well as smc02909 (transmembrane transglycosylase), trigger factor (tig) and smc00717 (probably ftsE) gave rise to strains that were unable to tolerate high salt and were uncompetitive for nodule occupancy relativ...

A competitive enzyme-linked immunosorbent assay (ELISA) was developed to quantify the amount of @-exotoxin from Bacillus thuringiensis in solution and to evaluate the ability of the antibodies to distinguish among various natural and synthetic compounds related to the 8-exotoxin. The &exotoxin was coupled to several proteins via glutaraldehyde, diazotization, and periodate procedures. The antib...

Seventy-five consecutive clinical Pseudomonas aeruginosa isolates were tested for in vitro exotoxin production. Exotoxin was demonstrated in culture filtrates biologically, by its ability to produce characteristic dermonecrotic lesions in guinea pigs, and seriologically, by counterimmunoelectrophoresis (CIE) with rabbit antiserum elicited with purified exotoxin. By these two methods, exotoxin w...

Pseudomonas aeruginosa exotoxin A is synthesized with a secretion signal peptide typical of proteins whose final destination is the periplasm. However, exotoxin A is released from the cell without a detectable periplasmic pool, suggesting that additional determinants in this protein are important for recognition by a specialized machinery of extracellular secretion. The role of the N terminus o...

In response to intraluminal challenge with crude cholera exotoxin, canine Thiry-Vella duodenal loops consistently produced isotonic fluid for a 24-36 hr period. Isotonic fluid production generally began within 15 min after challenge. Mean bicarbonate concentration of fluid produced by duodenal loops was 24+/-6 (SD) mEq/liter. Perfusion of exotoxin-treated duodenal loops with an isotonic electro...