in vitro methods provide a variety of tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, an efficient protocol was developed for in vitro propagation of colutea gifana, a rare and endangered plant species with limited reproductive capacity that grows in a narrow area of iran. single node explants were used for a serie...

The optimization of micropropagation for blueberries is crucial due to the growing blueberry industry and demand plantlets. This study focused on four stages: explant sterilization, in vitro establishment, proliferation, ex rooting, aiming establish an efficient propagation system southern highbush cultivar ‘ZY09’. most effective sterilization method was a 60 s treatment with 75% ethanol 5 min ...

The endangered medicinal plant Eryngium maritimum L. faces significant natural and anthropogenic threats. Therefore, in vitro propagation is recommended for both conservation commercial purposes. aim of the study was to develop a series protocols seed disinfection, multiplication rooting, ex field adaptation. For explant length temperature three consecutive disinfectants were investigated. Macr...

Abstract Ninebark is a very popular ornamental shrub. Micropropagation an efficient method for mass production of uniform plant material. This study was designed to develop and optimize conditions at all phases ninebark micropropagation. For the multiplication stage, Murashige Skoog (MS) medium full concentration pH 5.8 chosen as basal medium. Sorbitol proved more effective carbohydrate source ...

A high throughput regeneration protocol has been developed for Lagerstroemia speciosa through node explants under the regime of various plant growth regulators (PGRs). This can provide an alternative mode to seed-grown plants and minimize cost–time regeneration, significantly. Murashige Skoog (MS) medium containing combinations PGRs exhibited a marked stimulatory effect on morphogenesis. Of tri...

To evaluate the effect of plastic film coverage on ex vitro acclimatization arrow cane (Gynerium sagitatum Aubl.), shoots “Criolla”, Martinera” and “Costera” cultivars were in micropropagated a medium supplied with 6-Benzylaminopurine half them rooted 1-Naphtalene acetic acid. Rooted unrooted transplanted shade house fog irrigation, into trays (72 clusters per tray), using peat as substrate was...