Spermatozoal and seminal plasma lipids of fourteen individual bulls were separated by column chromatography into neutral lipid and several phospholipid fractions. Elution progress was monitored by thin-layer chromatography. Each phospholipid constituent was determined by phosphorus analysis. Total lipid, cholesterol and plasmalogen contents were determined. The fatty acids and aldehydes of the ...

Pure phosphatidyl ethanolamine and lecithin from egg yolks were fed to rats in saline or in olive oil and the changes in individual phospholipids in the intestinal wall, liver, and plasma of the animals were studied. Ingestion of olive oil alone produced increased levels of all phospholipid fractions in each of the three tissues. Feeding phosphatidyl ethanolamine in saline resulted in slightly ...

Summarv. Ethanolamine is readily metabolized by oat, pea, wheat, apple and carrot tissue preparations. Ethanolamine-1,2 14C was incorporated into the lipid fraction, and 14C activity was distributed in the organic acid, sugar, acid volatile, carbon dioxide and insoluble residule fractions. The distribution varied with the particular tissue. Incorporation into the lipid fraction occurred in tiss...

Summarv. Ethanolamine is readily metabolized by oat, pea, wheat, apple and carrot tissue preparations. Ethanolamine-1,2 14C was incorporated into the lipid fraction, and 14C activity was distributed in the organic acid, sugar, acid volatile, carbon dioxide and insoluble residule fractions. The distribution varied with the particular tissue. Incorporation into the lipid fraction occurred in tiss...

oxidation via the tricarboxylic acid cycle. The metabolism of [14C]ethanolamine to fatty acids via acetate has been shown to occur in mammals (Sprinson & Coulon, 1954; Sprinson & Weliky, 1969), but not previously ina bacterium. Thedecreased incorporation of [14C]ethanolamine that occurs when an alternative N source is present is probably related to the repression by (NH4)2S04 of the phospholyas...

The effects of eritadenine, a constituent of the Lentinus edodes mushroom, and ethanolamine, the base constituent of phosphatidylethanolamine (PE), on fatty acid desaturase activities and lipid profiles were investigated comparatively in rats. Rats were fed a control diet or a diet supplemented with either eritadenine (0.05 g/kg) or ethanolamine (8 g/kg) for 14 d. Eritadenine and ethanolamine h...

Ethanolamine kinase (ATP:ethanolamine O-phosphotransferase, EC 2.7.1. 82) catalyzes the committed step of phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway. The gene encoding ethanolamine kinase (EKI1) was identified from the Saccharomyces Genome Data Base (locus YDR147W) based on its homology to the Saccharomyces cerevisiae CKI1-encoded choline kinase, which also exhibits eth...

After the original demonstration by Stetten (1) that the nitrogen atom of DL-serine-N15 is converted to the nitrogen of ethanolamine in the intact rat, considerable evidence has accrued to indicate that in mammalian tissues L-serine is decarbosylated to ethanolamine. Not only are the B-carbon, B-hydrogen, and nitrogen of serine extensively utilized as a unit for the synthesis of the ethanolamin...

The conditions for maximal activity of ethanolamine phosphokinase from rat liver were determined. All of the activity was located in the supernatant fraction after centrifugation at Ioo ooo X g for 6o min. The enzyme activity had a pH opt imum at 8.5 and an apparent I(m for ethanolamine of I , IO 4 M at an ATP and Mg 2+ concentration of 3.o mM. The enzyme uses the Mg-ATP complex as substrate an...

Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to participate in the enzyme-catalyzed uridine diphosphate-galactose:lipopolysacchar...