A two-dimensional imaging system (Sniffer-camera) for visualizing the concentration distribution of ethanol vapor emitting from wine in a wine glass has been developed. This system provides image information of ethanol vapor concentration using chemiluminescence (CL) from an enzyme-immobilized mesh. This system measures ethanol vapor concentration as CL intensities from luminol reactions induce...

The effect of ethanol on the structure of DNA confined to mica in the presence of Mg2+was examined by varying the ethanol concentration and imaging the DNA by atomic force microscopy. Contour length measurements of the DNA show a transition from all-B-form at 0% ethanol to all-A-form at >25% ethanol. At intermediate ethanol concentrations, contour lengths suggest that individual molecules of ai...

chronic ethanol consumption leads to oxidative stress in the heart and erythrocytes of rats. as ziziphus jujuba fruit has been shown to have potent antioxidants, such as flavonoids, we conducted this study to evaluate the effects of aqueous fruit extracts from z. jujuba on rat hearts and erythrocytes following chronic ethanol consumption. twenty-eight male wistar rats were randomly divided into...

background: blood alcohol concentration is a major factor in forensic analysis in criminal and civil litigation. along with ethanol, other volatile compounds such as n-propanol are produced as products of putrefaction. the aim of this study is the evaluation of ethanol and n-propanol in postmortem blood. methods: a case-control study was done on 300 blood samples obtained from cadavers that wer...

Ethanol stimulated the leakage of amino acids and 260-nm-light-absorbing compounds from cells of Saccharomyces cerevisiae. The efflux followed first-order kinetics over an initial period. In the presence of lethal concentrations of ethanol, the efflux rates at 30 and 36 degrees C were an exponential function of ethanol concentration: k(e) = k(e)e, where k(e) and k(e) are the efflux rate constan...

Ethyl glucuronide (EtG) is a direct ethanol biomarker and U.S. Department of Health and Human Services has advised that specificity studies at low EtG levels are needed for distinction of ethanol consumption and incidental exposure. The authors report urinary EtG excretion with ethanol abstinence, dermal exposure and oral consumption. EtG concentration by sensitive liquid chromatography-tandem ...

Sir,—In a clinical evaluation of ethanol as an anaesthetic agent, Isaac and Dundee (1969) and Dundee and coworkers (1970) established that 8% w/v (10% v/v) ethanol in Hartmann's solution was the most satisfactory concentration for i.v. infusion in man and was associated with a low frequency of thrombophlebitis. Sanderson and co-workers (1970) confirmed that in vitro ethanol solutions in concent...

The enzymatic alcohol (ethanol, ALC) analytical pack for the duPont aca is intended primarily for the analysis of serum or plasma samples containing no visibly detectable free hemoglobin (non-hemolyzed samples). It has become apparent, through clinical and medicolegal consultation, that this methodology has been applied for assay of ethanol content in hemolyzed samples (sample sources: clinical...

This study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regardless of the temperature (5.0, 15.0, or 25.0 °C). The maximum recovery of cellulase activity as ...

Surface-engineered yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase on the cell surface was used for direct production of ethanol from uncooked raw starch. By using 50 g/L cells during batch fermentation, ethanol concentration could reach 53 g/L in 7 days. During repeated batch fermentation, the production of ethanol could be maintai...