conclusions our study emphasized the increasing role of esbl genes, especially blactx-m15 in epec isolates. results of 42 epec, eight isolates carried the blactx-m1. none of the isolates carried blactx-m2 and blactx-m9. the blactx-m15 variant was identified in all of blactx-m1-positive isolates. furthermore, blashv and blatem genes were detected in 40.5% (n = 17) and 19% (n = 8) of all epec iso...

conclusions the activities studied by the production of pnp were determined by following the increase at 410 nm. the maximum pnp was produced at 30°c with an optical density of 10.62 in the presence of cytoplasmic expression of oph (pet21a-opd). consequently, our results suggest cytoplasmic expression system as an appropriate candidate with a high amount of oph in spite of inclusion body format...

conclusions this study indicated the contamination of lettuce by stec strains and its possible role as the source of infection. resistance to both tetracycline and ampicillin may be considered as an emergency alarm for a multidrug resistance of stec strains. results eight samples were positive for presence of stec strains, three contained stx1, five contained stx2, and one sample was positive f...

results in this study, high resistances were found to nalidixic acid (59.7%) and gentamicin (52.3%) but high susceptibilities were found to ciprofloxacin (59.3%) and ceftriaxone (36.3%). conclusions according to the results obtained in this study ciprofloxacin is an effective antibiotic in e. coli infections. objectives the current study aimed to investigate the resistance of escherichia coli a...

background infectious diarrhoeal diseases cause major problems throughout the world and are responsible for considerable morbidity and mortality. enteropathogenic escherichia coli (epec) may cause infantile diarrhoea among children in developing countries. objectives the aim of this study was to define the prevalence of epec strains in raw milk samples. materials and methods raw milk samples co...

We analyzed the prevalence of diarrheagenic Escherichia coli (DEC) markers and common phenotypes in 2,164 E. coli isolates from 282 DEC-positive samples. Enteropathogenic E. coli (EPEC) and enteroaggregative E. coli (EAEC) were very diverse and were not correlated with diarrhea. Enterotoxigenic E. coli (ETEC) estA and enterohemorrhagic E. coli (EHEC) belonged to a few phenotypes and were signif...

Escherichia coli strains of serotype O51:H40 were studied with regard to the presence of several virulence properties and their genetic diversity and enteropathogenicity in rabbit ileal loops. This serotype encompasses potential enteropathogenic strains mostly classified as being atypical enteropathogenic E. coli (EPEC) strains, which are genetically closer to enterohemorrhagic E. coli than to ...

Enterohemorrhagic Escherichia coli (EHEC) with serotype O157:H7 is a major foodborne pathogen. Here, we report the draft genome sequence of EHEC O157:H7 strain MC2 isolated from cattle in France. The assembly contains 5,400,376 bp that encoded 5,914 predicted genes (5,805 protein-encoding genes and 109 RNA genes).

introduction: chitosan possesses more than 5000 glucossamine residue. since chitosan possesses potentially reactive amino functional groups, this polymer has been shown to be of potential use in many different fields, including as an antifungal compound in agriculture, flocculating in waste water treatment, a food additive and alimentary industries, hydrating agent in cosmetics and more recentl...

We investigated the prevalence of the virulence genes specific for five major pathogroups of diarrheagenic Escherichia coli (DEC) in primary cultures from feces of animals slaughtered for human consumption in Burkina Faso. For the study, 704 feces samples were collected from cattle (n = 304), chickens (n = 350), and pigs (n = 50) during carcass processing. The presence of the virulence-associat...