1,3,5(10),16-Estratetraen-3-01 (estratetraenol) that has previotisly been found in the urine of women in late pregnancy and proved to be biosynthesized in human placenta from 4,16-androstadien-3-one, a hepatic metabolite of testosterone, was converted under aerobic conditions by female rat liver microsomes into estratetraenol 16a,l7a-epoxide, estratetraenol 16P,17P-epoxide, 16,17-epiestriol, an...

The stereochemistry of diol epoxide formation in mouse epidermis upon topical applicationof [3H]-l/f,2A-dihydroxy-l,2-dihydro-5-methylchrysene (|3H|-5-MeC-l/f,2A-diol)and |3Hl-6-MeC-lÄ,2Ä-diol, and the tumorigenicityin mouse skin and in newborn mice of the R,S,S,R and S,R,K,S enantiomers of l,2-dihydroxy-3,4-epoxy-l,2,3,4-tetrahydro-5methylchrysene (5-MeC-l,2-diol-3,4-epoxide), 5-MeC-7,8-diol...

leishmaniasis has a wide spectrum of signs and symptoms due to infection to numbers of leishmania species and makes enormous mortality and morbidity. there are clues of antileishmanial effects of prenylated coumarins. apiaceae family is one of the most important sources of coumarins. air-dried aerial parts of ferulago angulata and fruits of prangos asperula were extracted with n -hexane, using ...

We have utilized a DNA clone complementary to epoxide hydrolase mRNA as a probe to examine the level of the mRNA in persistent hepatocyte nodules and hepatomas induced by the Solt-Farber chemical carcinogenesis procedure. Epoxide hydrolase mRNA is increased 14-fold in nodules as compared to the level in normal liver. When rats with liver nodules were administered phenobarbital, an inducer of ep...

Epoxide carboxylase from Xanthobacter strain Py2 catalyzes the reductant- and NAD+-dependent carboxylation of aliphatic epoxides to beta-keto acids. Epoxide carboxylase from Xanthobacter strain Py2 has been resolved from cell extracts by anion-exchange chromatography into three protein components, designated I, II, and III, that are obligately required for functional reconstitution of epoxide c...

The epoxide hydrolase gene from Agrobacterium radiobacter AD1, a bacterium that is able to grow on epichlorohydrin as the sole carbon source, was cloned by means of the polymerase chain reaction with two degenerate primers based on the N-terminal and C-terminal sequences of the enzyme. The epoxide hydrolase gene coded for a protein of 294 amino acids with a molecular mass of 34 kDa. An identica...

A growing body of evidence implicates epoxide metabolites of mutagenic and carcinogenic polycyclic hydrocarbons as either the only species, or one of the contributing species responsible for these adverse effects. Selective induction of epoxide hydratase(s) catalyzing the transformation of epoxides to electrophilically unreactive dihydrodiols, under conditions not leading to increases in monoox...

Epoxide hydrolases catalyze hydrolytic epoxide ring-opening, most often via formation of a covalent hydroxyalkyl-enzyme intermediate. A mutant of Agrobacterium radiobacter epoxide hydrolase, in which the phenylalanine residue that flanks the invariant catalytic aspartate nucleophile is replaced by a threonine, exhibited inactivation during conversion when the (R)-enantiomer of para-nitrostyrene...

s for posters:Experimental Biology 2008, San Diego, April 2008IDENTIFICATION OF A NEW POTENTIAL HUMAN EPOXIDE HYDROLASE(NEH2)Martina Decker, Magdalena Adamska, Michael Arand. Leopoldina Symposium of Lipid Signaling, Frankfurt, September 2008CHARACTERIZATION OF A NOVEL HUMAN EPOXIDE HYDROLASE (EH3)CAPABLE OF CONVERTING EPOXYEICOSATRIENOIC ACIDS (EETS)Martina Decker, M...

Epoxide hydrolases catalyze hydrolytic epoxide ringopening, most often via formation of a covalent hydroxyalkyl-enzyme intermediate. A mutant of Agrobacterium radiobacter epoxide hydrolase, in which the phenylalanine residue that flanks the invariant catalytic aspartate nucleophile is replaced by a threonine, exhibited inactivation during conversion when the (R)-enantiomer of para-nitrostyrene ...