Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasm...

The indiscriminate use of commercial antimicrobial drugs has resulted in multiple drug resistance and adverse effects on the host. The present study was aimed to elucidate the effect of different biotic/abiotic elicitors on the growth as well as to enhance the metabolite production in leaf and epicotyl calli of Nigella sativa. Further, antibacterial activity of these callus extracts was evaluat...

the present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (pgrs) combinations under in vitro conditions. the cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in ms medium containing ba (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and tdz (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...

Bowman-Birk soybean trypsin inhibitor (BBSTI) but not Kunitz soybean trypsin inhibitor (KSTI) was found in samples of undifferentiated and partially differentiated Amsoy 71 tissue culture callus. This suggests the differential metabolism of these two classes of proteinase inhibitors, whether the difference be in synthesis, in rates of degradation, or both. The differential metabolism of the pro...

An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...

Autoregulation of nodulation (AON) is a long-distance signalling regulatory system maintaining the balance of symbiotic nodulation in legume plants. However, the intricacy of internal signalling and absence of flux and biochemical data, are a bottleneck for investigation of AON. To address this, a new computational modelling approach called "Computational Complementation" has been developed. Th...

The ramosus (rms) mutation (rms1) of pea (Pisum sativum) causes increased branching through modification of graft-transmissible signal(s) produced in rootstock and shoot. Additional grafting techniques have led us to propose that the novel signal regulated by Rms1 moves acropetally in shoots and acts as a branching inhibitor. Epicotyl interstock grafts showed that wild-type (WT) epicotyls graft...

An apparatus for measuring simultaneously the surface electrical potentials along epicotyl was developed to study the largely bending situation by gravity. Potentials increased on the upper side and decreased on the lower side after the horizontal placement. The time course of electrical changes consisted of two components which correspond to growth movements observed during gravitropism.