The present work arose out of an attempt to understand the course of cellulose breakdown in preparations of cellulolytic enzymes which also contained cellobiase activity. Previous investigations of the ,B-glucosidases have been of three kinds-an examination of their specificity ranges (reviewed by Veibel, 1950), the demonstration of their reversibility and the study of glucosyl transfer. Croft-...

The kinetic parameters for inorganic phosphate and inorganic arsenate as substrates for the synthesis of ATP and ADP-arsenate, respectively, by beef heart submitochondrial particles have been determined. The Vm and Km values for arsenate and phosphate, as well as the Km values for ADP in the two reactions, are the same within experimental error of the measurements. These data are consistent wit...

1. An in vitro method which used enzymic digestion of the food matrix to release biologically available vitamin B6 is described. 2. Vitamin B6-fortified liquid model foods were thermally processed. After these foods had been freeze-dried, one part was subjected to enzymic hydrolysis at pH 2.0 with pepsin (EC 3.4.23.1) followed by a hydrolysis at pH 8.0 with pancreatin. The vitamins that were fo...

The strong acids that are required for complete hydrolysis of proteins destroy some amino acids, such as tryptophan, asparagine, and glutamine. Other amino acids are destroyed to a lesser extent or in certain cases are released incompletely from peptide linkage. It is evident that these difficulties, as well as others, could be overcome by employing proteolytic enzymes as the sole agents for hy...

The esterases of animal tissues have generally been characterized by their substrate specificity and by their behaviour towards various inhibitors. The irreversible inhibition by organic phosphates, which is due to phosphorylation of the active group (Burgen, 1949; Wilson & Bergmann, 1950a; Aldridge, 1953d), is a common property of most esterases, an interesting exception being the Aesterase of...

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