A light-addressable potentiometric (silicon) sensor was used in an immunofiltration procedure for the detection of pathogenic bacteria. Yersinia pestis was detected by filtering the cells onto nitrocellulose membranes and then filtering anti-Y. pestis mouse monoclonal antibody and anti-mouse immunoglobulin G-horseradish peroxidase conjugate. For Neisseria meningitidis detection, mouse monoclona...

Some enzymes were immobilized in an inner layer of a filter paper using the osmotic action of a contact dehydrating sheet (CDS). CDS was examined to determine if the efficiency and rapidity of an enzyme reaction could be improved, thereby making better of the preparation of an immobilized-enzyme membrane. The activity of the membrane, in which an enzyme exists in its layer, was compared with th...

Sensors capable of quantitative real-time measurements may present the easiest and most accurate way to study enzyme activities. Here we present a novel DNA-based sensor for specific and quantitative real-time measurement of the enzymatic activity of the essential human enzyme, topoisomerase I. The basic design of the sensor relies on two DNA strands that hybridize to form a hairpin structure w...

Neuraminidase inhibitor assay requiring complicated step-by-step operation was successfully integrated into ”Single-Step” by using CPC sensor. We developed a combinable PDMS capillary (CPC) sensor for enzyme inhibition assay using the concave and convex type PDMS substrates. In the conventional enzyme inhibitor assay, complicated analytical procedures, such as mixing and dissolution, are requir...

The reproducible degradation of thin polymer films in the presence of an analyte or a reaction product of an analyte can be utilized to produce highly sensitive, disposable biosensors [1, 2]. This type of sensor has been particular useful for the detection of enzyme concentrations. Recent work in this area lead to the development of a sensor for α-chymotrypsin based on the changes in the Surfac...

A reusable extended-gate field-effect transistor (FET) sensor with an 11-ferrocenyl-1-undecanethiol (11-FUT) modified gold electrode was developed for applying to enzyme immunoassay. It was found that the 11-FUT modified FET sensor detected a thiol compound 50 times or more repeatedly after a treatment with a 5% hydrogen peroxide solution. The gate-voltage shift of the FET sensor showed a fairl...

An amperometric enzyme-based sensor-system for the direct detection of formaldehyde in air is under investigation. The biosensor is based on a native bacterial NAD(+)- and glutathione-independent formaldehyde dehydrogenase as biorecognition element. The enzyme was isolated from Hyphomicrobium zavarzinii strain ZV 580, grown on methylamine hydrochloride in a fed-batch process. The sensor depends...

This study aims to characterize the tungsten-urea analyte indicator electrode. The method used is biosensor potentiometry with urease enzyme immobilization technique. electrode was coated PVA-enzyme glutaraldehyde (GA) 2.9% PVC-KTpClPB- o-NPOE variation of 61% and 66%. Characterization electrodes using SEM-EDS, FTIR XRD analysis. A1-4 sample 1x 1x, 61%. A3-4 3x Likewise, reasoning samples 66% T...