We report a method for immobilizing glucose dehydrogenase on the inside surface of nylon tubes to produce an immobilized-enzyme nylon-tube reactor. The glucose dehydrogenase reactor is integrated into the flow system of a continuous-flow analyzer to facilitate routine analysis of serum glucose at 50 samples/h. We compared results with those by the reference hexokinase/glucose-6-phosphate dehydr...

Fatty acid butyl esters were synthesized from sunflower oil with 1-butanol using a homogeneous Rhizomucor miehei lipase in a biphasic organic (triglyceride, 1-butanol, hexane)- water (with enzyme) system in a continuous setup consisting of a cascade of a stirred tank reactor and a continuous centrifugal contactor separator (CCCS), the latter being used for integrated reaction and liquid-liquid ...

The present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. The effect of operating parameters, impellers speed, operational period, temperature, pH and scale-up were considered.The acetic acid pH: 5.2 increased the leaching of lipoxygenase- 1.The sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has bee...

BACKGROUND To make lignocellulosic fuel ethanol economically competitive with fossil fuels, it is necessary to reduce the production cost. One way to achieve this is by increasing the substrate concentration in the production process, and thus reduce the energy demand in the final distillation of the fermentation broth. However, increased substrate concentration in simultaneous saccharification...

Penicillin acylase, an industrially important biocatalyst catalyzes the conversion of penicillins to 6-amino penicillanic acid (6-APA) which is the main precursor for the production of semi-synthetic -lactam antibiotics. The present work involves the continuous production of 6-APA in a packed column reactor by using agarose immobilized penicillin acylase as a block polymer. The strain Escherich...

Galacto-oligosaccharides (GOS), increasingly popular prebiotics, are synthesized by enzymatic conversion of lactose. Among others, the total production costs significantly influenced costly enzyme. Therefore, it was investigated if reuse and full recovery enzyme is feasible, followed development a semi-continuous process in order to maintain consistent high GOS yield. As preliminary step, enzym...

Present study highlights on isolation of bioactive peptide from the sesame protein hydrolysate with an objective of better utilization sesame meal. Sesame protein isolate was enzymatically modified to prepare sesame protein hydrolysate using enzyme ‘Protease A Amano 2G’ in an enzyme membrane reactor and the hydrolysate was subsequently fractionated by ultrafiltration using membranes of decreasi...

A raptd flow-mlectron sandwtch enzyme immunoassay suitabIe for the direct determmatron of proteins m btoIogtcaI sampIes rs described.. ‘The proposed system utihzes fugfify active adenosme deammase-antibody coqugates m conlunctron with a flow-through unmunoreactor and an ammomum ion-sefectrve potentrometnc detector. After appropnate sampfe/.reagent mlectton steps, the enzyme actrvtty bound to th...